5UQW
Crystal structure of human KRAS G12V mutant in complex with GDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-27 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.075 |
Spacegroup name | P 3 |
Unit cell lengths | 84.344, 84.344, 41.809 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.690 - 1.500 |
R-factor | 0.11847 |
Rwork | 0.117 |
R-free | 0.15272 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.016 |
RMSD bond angle | 1.676 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.161 | 0.239 |
Number of reflections | 52602 | |
<I/σ(I)> | 8.6 | 5.7 |
Completeness [%] | 98.6 | 90.5 |
Redundancy | 4.3 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M Bis-Tris, 25% w/v PEG3350, pH 6.5 |