5UM9
Flap endonuclease 1 (FEN1) D86N with 5'-flap substrate DNA and Sm3+
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Flap endonuclease 1 | polymer | 341 | 38510.2 | 1 | UniProt (P39748) Pfam (PF00752) Pfam (PF00867) | Homo sapiens (Human) | FEN-1,DNase IV,Flap structure-specific endonuclease 1,Maturation factor 1,hFEN-1 |
| 2 | B (D) | DNA (5'-D(*AP*CP*TP*CP*TP*GP*CP*CP*TP*CP*AP*AP*GP*AP*CP*GP*GP*T)-3') | polymer | 18 | 5476.6 | 1 | synthetic construct | ||
| 3 | C (E) | DNA (5'-D(P*TP*CP*TP*TP*GP*AP*GP*GP*CP*AP*GP*AP*GP*T)-3') | polymer | 17 | 5266.4 | 1 | synthetic construct | ||
| 4 | D (F) | DNA (5'-D(*AP*CP*CP*GP*TP*CP*C)-3') | polymer | 7 | 2058.4 | 1 | synthetic construct | ||
| 5 | E (A) | POTASSIUM ION | non-polymer | 39.1 | 1 | Chemie (K) | |||
| 6 | F, G, H, I, J (A) | SAMARIUM (III) ION | non-polymer | 150.4 | 5 | Chemie (SM) | |||
| 7 | K, L, M, N (A, D, E, F) | water | water | 18.0 | 35 | Chemie (HOH) |
Sequence modifications
A: 2 - 336 (UniProt: P39748)
| PDB | External Database | Details |
|---|---|---|
| Asn 86 | Asp 86 | engineered mutation |
| Leu 337 | - | expression tag |
| Glu 338 | - | expression tag |
| Val 339 | - | expression tag |
| Leu 340 | - | expression tag |
| Phe 341 | - | expression tag |
| Gln 342 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 51311.6 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 790.9 | |
| All* | Total formula weight | 52102.5 |
