5ULF

Crystal Structure of a UbcH5b~Ub conjugate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	Ubiquitin-conjugating enzyme E2 D2	polymer	152	17214.6	2	UniProt (P62837) Pfam (PF00179) In PDB	Homo sapiens (Human)	(E3-independent) E2 ubiquitin-conjugating enzyme D2,E2 ubiquitin-conjugating enzyme D2,Ubiquitin carrier protein D2,Ubiquitin-conjugating enzyme E2(17)KB 2,Ubiquitin-conjugating enzyme E2-17 kDa 2,Ubiquitin-protein ligase D2,p53-regulated ubiquitin-conjugating enzyme 1
2	B, D	Ubiquitin	polymer	76	8576.8	2	UniProt (P0CG47) Pfam (PF00240) In PDB	Homo sapiens (Human)
3	A, C	GLYCEROL	non-polymer		92.1	5	Chemie (GOL)
4		water	water		18.0	251	Chemie (HOH)

Sequence modifications

A, C: 1 - 147 (UniProt: P62837)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Leu -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Ser 21	Cys 21	engineered mutation
Arg 22	Ser 22	engineered mutation
Lys 85	Cys 85	engineered mutation
Ser 107	Cys 107	engineered mutation
Ser 111	Cys 111	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	51582.9
Non-Polymers*	Number of molecules	5
	Total formula weight	460.5
All*	Total formula weight	52043.4

*Water molecules are not included.