5ULF
Crystal Structure of a UbcH5b~Ub conjugate
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000151 | cellular_component | ubiquitin ligase complex |
| A | 0000209 | biological_process | protein polyubiquitination |
| A | 0004842 | molecular_function | ubiquitin-protein transferase activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005524 | molecular_function | ATP binding |
| A | 0005634 | cellular_component | nucleus |
| A | 0005654 | cellular_component | nucleoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0006511 | biological_process | ubiquitin-dependent protein catabolic process |
| A | 0016567 | biological_process | protein ubiquitination |
| A | 0016740 | molecular_function | transferase activity |
| A | 0019787 | molecular_function | ubiquitin-like protein transferase activity |
| A | 0031371 | cellular_component | ubiquitin conjugating enzyme complex |
| A | 0031625 | molecular_function | ubiquitin protein ligase binding |
| A | 0032991 | cellular_component | protein-containing complex |
| A | 0036211 | biological_process | protein modification process |
| A | 0051865 | biological_process | protein autoubiquitination |
| A | 0061630 | molecular_function | ubiquitin protein ligase activity |
| A | 0061631 | molecular_function | ubiquitin conjugating enzyme activity |
| A | 0070062 | cellular_component | extracellular exosome |
| A | 0070936 | biological_process | protein K48-linked ubiquitination |
| C | 0000151 | cellular_component | ubiquitin ligase complex |
| C | 0000209 | biological_process | protein polyubiquitination |
| C | 0004842 | molecular_function | ubiquitin-protein transferase activity |
| C | 0005515 | molecular_function | protein binding |
| C | 0005524 | molecular_function | ATP binding |
| C | 0005634 | cellular_component | nucleus |
| C | 0005654 | cellular_component | nucleoplasm |
| C | 0005829 | cellular_component | cytosol |
| C | 0006511 | biological_process | ubiquitin-dependent protein catabolic process |
| C | 0016567 | biological_process | protein ubiquitination |
| C | 0016740 | molecular_function | transferase activity |
| C | 0019787 | molecular_function | ubiquitin-like protein transferase activity |
| C | 0031371 | cellular_component | ubiquitin conjugating enzyme complex |
| C | 0031625 | molecular_function | ubiquitin protein ligase binding |
| C | 0032991 | cellular_component | protein-containing complex |
| C | 0036211 | biological_process | protein modification process |
| C | 0051865 | biological_process | protein autoubiquitination |
| C | 0061630 | molecular_function | ubiquitin protein ligase activity |
| C | 0061631 | molecular_function | ubiquitin conjugating enzyme activity |
| C | 0070062 | cellular_component | extracellular exosome |
| C | 0070936 | biological_process | protein K48-linked ubiquitination |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 6 |
| Details | binding site for residue GOL A 201 |
| Chain | Residue |
| A | LYS66 |
| A | VAL67 |
| A | SER83 |
| A | ILE84 |
| A | ARG90 |
| A | HOH332 |
| site_id | AC2 |
| Number of Residues | 7 |
| Details | binding site for residue GOL A 202 |
| Chain | Residue |
| A | THR129 |
| A | ASP130 |
| A | ARG131 |
| A | GLU132 |
| A | ARG72 |
| A | SER80 |
| A | ASN81 |
| site_id | AC3 |
| Number of Residues | 6 |
| Details | binding site for residue GOL C 201 |
| Chain | Residue |
| C | LYS66 |
| C | VAL67 |
| C | SER83 |
| C | ILE84 |
| C | ARG90 |
| C | HOH322 |
| site_id | AC4 |
| Number of Residues | 6 |
| Details | binding site for residue GOL C 202 |
| Chain | Residue |
| C | LEU13 |
| C | ARG22 |
| C | ALA23 |
| C | HOH302 |
| C | HOH329 |
| C | HOH338 |
| site_id | AC5 |
| Number of Residues | 1 |
| Details | binding site for residue GOL C 203 |
| Chain | Residue |
| C | TYR145 |
Functional Information from PROSITE/UniProt
| site_id | PS00299 |
| Number of Residues | 26 |
| Details | UBIQUITIN_1 Ubiquitin domain signature. KakIqDkegIPpdqQrLIFaGkqleD |
| Chain | Residue | Details |
| B | LYS27-ASP52 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | SITE: Interacts with activating enzyme |
| Chain | Residue | Details |
| B | ARG54 | |
| B | ARG72 | |
| D | ARG54 | |
| D | ARG72 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | SITE: Essential for function |
| Chain | Residue | Details |
| B | HIS68 | |
| D | HIS68 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphoserine; by PINK1 => ECO:0000269|PubMed:24660806, ECO:0000269|PubMed:24751536, ECO:0000269|PubMed:24784582, ECO:0000269|PubMed:25527291 |
| Chain | Residue | Details |
| B | SER65 | |
| D | SER65 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 2 |
| Details | MOD_RES: (Microbial infection) ADP-ribosylthreonine => ECO:0000269|PubMed:32330457 |
| Chain | Residue | Details |
| B | THR66 | |
| D | THR66 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | MOD_RES: ADP-ribosylglycine => ECO:0000269|PubMed:28525742 |
| Chain | Residue | Details |
| B | GLY76 | |
| D | GLY76 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 2 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16443603 |
| Chain | Residue | Details |
| B | LYS6 | |
| D | LYS6 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 4 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins) |
| Chain | Residue | Details |
| B | GLY76 | |
| D | GLY76 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 4 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16443603, ECO:0000269|PubMed:16543144 |
| Chain | Residue | Details |
| B | LYS11 | |
| B | LYS48 | |
| D | LYS11 | |
| D | LYS48 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 2 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000305|PubMed:15466860 |
| Chain | Residue | Details |
| B | LYS27 | |
| D | LYS27 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 2 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16543144, ECO:0000269|PubMed:25752573, ECO:0000269|PubMed:25752577, ECO:0000269|PubMed:34239127 |
| Chain | Residue | Details |
| B | LYS29 | |
| D | LYS29 |
| site_id | SWS_FT_FI11 |
| Number of Residues | 2 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:25752577 |
| Chain | Residue | Details |
| B | LYS33 | |
| D | LYS33 |
| site_id | SWS_FT_FI12 |
| Number of Residues | 2 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16543144, ECO:0000269|PubMed:18719106 |
| Chain | Residue | Details |
| B | LYS63 | |
| D | LYS63 |
