5UIO

structure of DHFR with bound DAP, p-ABG and NADP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E (A, B, C, D, E)	Dihydrofolate reductase	polymer	170	19207.6	5	UniProt (P0ABQ4) Pfam (PF00186)	Escherichia coli
2	F, I, L, Q, U (A, B, C, D, E)	PYRIMIDINE-2,4-DIAMINE	non-polymer		110.1	5	Chemie (LG3)
3	G, M, R, V (A, C, D, E)	N-(4-aminobenzene-1-carbonyl)-L-glutamic acid	non-polymer		266.3	4	Chemie (8DM)
4	H, N, S, W (A, C, D, E)	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	non-polymer		743.4	4	Chemie (NAP)
5	J, O, T, X (B, C, D, E)	BETA-MERCAPTOETHANOL	non-polymer		78.1	4	Chemie (BME)
6	K, P (B, C)	FORMIC ACID	non-polymer		46.0	2	Chemie (FMT)
7	AA, BA, CA, Y, Z (C, D, E, A, B)	water	water		18.0	616	Chemie (HOH)

Sequence modifications

A, B, C, D, E: 1 - 159 (UniProt: P0ABQ4)

PDB	External Database	Details
Met 0	-	initiating methionine
Gly 160	-	expression tag
Gly 161	-	expression tag
Gly 162	-	expression tag
Gly 163	-	expression tag
His 164	-	expression tag
His 165	-	expression tag
His 166	-	expression tag
His 167	-	expression tag
His 168	-	expression tag
His 169	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	96038.1
Non-Polymers*	Number of molecules	19
	Total formula weight	4993.8
All*	Total formula weight	101031.9

*Water molecules are not included.