5UGX

Crystal Structure of the Tyrosine Kinase Domain of FGF Receptor 2 Harboring a E565A/D650V double Gain-of-Function Mutation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (B, A)	Fibroblast growth factor receptor 2	polymer	324	37036.6	2	UniProt (P21802) Pfam (PF07714)	Homo sapiens (Human)	FGFR-2,K-sam,KGFR,Keratinocyte growth factor receptor
2	C, D, G, H (B, A)	SULFATE ION	non-polymer		96.1	4	Chemie (SO4)
3	E, I (B, A)	PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER	non-polymer		505.2	2	Chemie (ACP)
4	F, J (B, A)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
5	K, L (B, A)	water	water		18.0	38	Chemie (HOH)

Sequence modifications

B, A: 458 - 768 (UniProt: P21802)

PDB	External Database	Details
Met 445	-	initiating methionine
Gly 446	-	expression tag
Ser 447	-	expression tag
Ser 448	-	expression tag
His 449	-	expression tag
His 450	-	expression tag
His 451	-	expression tag
His 452	-	expression tag
His 453	-	expression tag
His 454	-	expression tag
Ser 455	-	expression tag
Gln 456	-	expression tag
Asp 457	-	expression tag
Ala 491	Cys 374	engineered mutation
Ala 565	Glu 448	engineered mutation
Val 650	Asp 533	conflict

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	74073.2
Non-Polymers*	Number of molecules	8
	Total formula weight	1443.3
All*	Total formula weight	75516.5

*Water molecules are not included.