5UAJ

Escherichia coli RNA polymerase RpoB S531L mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, G, H (A, B, G, H)	DNA-directed RNA polymerase subunit alpha	polymer	329	36558.7	4	UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118)	Escherichia coli (strain K12)	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
2	C, I (C, I)	DNA-directed RNA polymerase subunit beta	polymer	1342	150847.0	2	UniProt (P0A8V2) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562)	Escherichia coli (strain K12)	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
3	D, J (D, J)	DNA-directed RNA polymerase subunit beta'	polymer	1407	155366.8	2	UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998)	Escherichia coli (strain K12)	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
4	E, K (E, K)	DNA-directed RNA polymerase subunit omega	polymer	91	10249.5	2	UniProt (P0A800) Pfam (PF01192)	Escherichia coli (strain K12)	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
5	F, L (F, L)	RNA polymerase sigma factor RpoD	polymer	613	70352.2	2	UniProt (P00579) Pfam (PF03979) Pfam (PF00140) Pfam (PF04546) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545)	Escherichia coli (strain K12)	Sigma-70
6	M, P (D, J)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
7	N, O, Q, R (D, J)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)

Sequence modifications

C, I: 1 - 1342 (UniProt: P0A8V2)

PDB	External Database	Details
Leu 531	Ser 531	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	919865.8
Non-Polymers*	Number of molecules	6
	Total formula weight	310.2
All*	Total formula weight	920176.0

*Water molecules are not included.