5UAJ
Escherichia coli RNA polymerase RpoB S531L mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-03-21 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97690 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 187.219, 204.426, 310.592 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.959 - 3.915 |
R-factor | 0.2607 |
Rwork | 0.260 |
R-free | 0.30730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4yg2 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.950 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.970 |
High resolution limit [Å] | 3.900 | 3.900 |
Number of reflections | 86257 | |
<I/σ(I)> | 11.6 | |
Completeness [%] | 81.3 | |
Redundancy | 5.6 | |
CC(1/2) | 0.634 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 300 | 0.1 M HEPES-HCl (pH 6.7), 0.2 M Ca Acetate, 30% PEG400, 10 mM DTT |