5U81

Acid ceramidase (ASAH1, aCDase) from naked mole rat, Cys143Ala, uncleaved

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Acid ceramidase isoform b	polymer	384	44157.5	1	UniProt (A0A0P6JG37) Pfam (PF15508) Pfam (PF02275) UniProt (by SIFTS) (G5AP74) In PDB	Heterocephalus glaber (Naked mole rat)
2	B	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1057.0	1	In PDB GlyTouCan (G82348BZ)
3	C	alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		910.8	1	In PDB GlyTouCan (G10756ZZ)
4	D	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
5	A	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
6	A	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
7	A	TETRAETHYLENE GLYCOL	non-polymer		194.2	1	Chemie (PG4)
8	A	PENTAETHYLENE GLYCOL	non-polymer		238.3	1	Chemie (1PE)
9		water	water		18.0	303	Chemie (HOH)

Sequence modifications

A: 22 - 395 (UniProt: A0A0P6JG37)

PDB	External Database	Details
Asp 12	-	expression tag
Arg 13	-	expression tag
His 14	-	expression tag
His 15	-	expression tag
His 16	-	expression tag
His 17	-	expression tag
His 18	-	expression tag
His 19	-	expression tag
Lys 20	-	expression tag
Leu 21	-	expression tag
Ala 143	Cys 143	engineered mutation
Ser 348	Asn 348	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	44157.5
Branched	Number of molecules	3
	Total formula weight	2392.2
Non-Polymers*	Number of molecules	6
	Total formula weight	687.6
All*	Total formula weight	47237.3

*Water molecules are not included.