5U4I

Structural Basis of Co-translational Quality Control by ArfA and RF2 Bound to Ribosome

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	23S rRNA	polymer	2904	941761.6	1	GenBank (42756)	Escherichia coli
2	B (B)	5S rRNA	polymer	118	38177.8	1	GenBank (1036415628)	Escherichia coli
3	C (C)	50S ribosomal protein L2	polymer	273	29923.6	1	UniProt (P60422) Pfam (PF00181) Pfam (PF03947)	Escherichia coli (strain K12)
4	D (D)	50S ribosomal protein L3	polymer	209	22277.5	1	UniProt (P60438) Pfam (PF00297)	Escherichia coli (strain K12)
5	E (E)	50S ribosomal protein L4	polymer	201	22121.6	1	UniProt (P60723) Pfam (PF00573)	Escherichia coli (strain K12)
6	F (F)	50S ribosomal protein L5	polymer	179	20333.6	1	UniProt (P62399) Pfam (PF00281) Pfam (PF00673)	Escherichia coli (strain K12)
7	G (G)	50S ribosomal protein L6	polymer	177	18932.8	1	UniProt (P0AG55) Pfam (PF00347)	Escherichia coli (strain K12)
8	H (H)	50S ribosomal protein L9	polymer	149	15789.0	1	UniProt (P0A7R1) Pfam (PF01281) Pfam (PF03948)	Escherichia coli (strain K12)
9	I (J)	50S ribosomal protein L11	polymer	142	14894.4	1	UniProt (P0A7J7) Pfam (PF03946) Pfam (PF00298)	Escherichia coli (strain K12)
10	J (K)	50S ribosomal protein L13	polymer	142	16050.6	1	UniProt (P0AA10) Pfam (PF00572)	Escherichia coli (strain K12)
11	K (L)	50S ribosomal protein L14	polymer	123	13565.1	1	UniProt (P0ADY3) Pfam (PF00238)	Escherichia coli (strain K12)
12	L (M)	50S ribosomal protein L15	polymer	144	15008.5	1	UniProt (P02413) Pfam (PF00828)	Escherichia coli (strain K12)
13	M (N)	50S ribosomal protein L16	polymer	136	15312.3	1	UniProt (P0ADY7) Pfam (PF00252)	Escherichia coli (strain K12)
14	N (O)	50S ribosomal protein L17	polymer	127	14393.7	1	UniProt (P0AG44) Pfam (PF01196)	Escherichia coli (strain K12)
15	O (P)	50S ribosomal protein L18	polymer	117	12794.7	1	UniProt (P0C018) Pfam (PF00861)	Escherichia coli (strain K12)
16	P (Q)	50S ribosomal protein L19	polymer	115	13159.3	1	UniProt (P0A7K6) Pfam (PF01245)	Escherichia coli (strain K12)
17	Q (R)	50S ribosomal protein L20	polymer	118	13528.0	1	UniProt (P0A7L3) Pfam (PF00453)	Escherichia coli (strain K12)
18	R (S)	50S ribosomal protein L21	polymer	103	11586.4	1	UniProt (P0AG48) Pfam (PF00829)	Escherichia coli (strain K12)
19	S (T)	50S ribosomal protein L22	polymer	110	12253.4	1	UniProt (P61175) Pfam (PF00237)	Escherichia coli (strain K12)
20	T (U)	50S ribosomal protein L23	polymer	100	11222.2	1	UniProt (P0ADZ0) Pfam (PF00276)	Escherichia coli (strain K12)
21	U (V)	50S ribosomal protein L24	polymer	104	11339.3	1	UniProt (P60624) Pfam (PF00467) Pfam (PF17136)	Escherichia coli (strain K12)
22	V (W)	50S ribosomal protein L25	polymer	94	10713.5	1	UniProt (P68919) Pfam (PF01386)	Escherichia coli (strain K12)
23	W (X)	50S ribosomal protein L27	polymer	85	9146.5	1	UniProt (P0A7L8) Pfam (PF01016)	Escherichia coli (strain K12)
24	X (Y)	50S ribosomal protein L28	polymer	78	9027.6	1	UniProt (P0A7M2) Pfam (PF00830)	Escherichia coli (strain K12)
25	Y (Z)	50S ribosomal protein L29	polymer	63	7286.5	1	UniProt (P0A7M6) Pfam (PF00831)	Escherichia coli (strain K12)
26	Z (0)	50S ribosomal protein L30	polymer	59	6554.8	1	UniProt (P0AG51) Pfam (PF00327)	Escherichia coli
27	AA (1)	50S ribosomal protein L32	polymer	57	6463.4	1	UniProt (P0A7N4) Pfam (PF01783)	Escherichia coli
28	BA (2)	50S ribosomal protein L33	polymer	55	6388.6	1	UniProt (P0A7N9) Pfam (PF00471)	Escherichia coli
29	CA (3)	50S ribosomal protein L34	polymer	46	5397.5	1	UniProt (P0A7P5) Pfam (PF00468)	Escherichia coli
30	DA (4)	50S ribosomal protein L35	polymer	65	7313.0	1	UniProt (P0A7Q1) Pfam (PF01632)	Escherichia coli	Ribosomal protein A
31	EA (5)	50S ribosomal protein L36	polymer	38	4377.4	1	UniProt (P0A7Q6) Pfam (PF00444)	Escherichia coli	Ribosomal protein B
32	FA (a)	16S rRNA	polymer	1533	497044.8	1	GenBank (817573384)	Escherichia coli
33	GA (b)	30S ribosomal protein S2	polymer	241	26781.7	1	UniProt (P0A7V0) Pfam (PF00318)	Escherichia coli
34	HA (c)	30S ribosomal protein S3	polymer	233	26031.3	1	UniProt (P0A7V3) Pfam (PF07650) Pfam (PF00189)	Escherichia coli
35	IA (d)	30S ribosomal protein S4	polymer	206	23514.2	1	UniProt (P0A7V8) Pfam (PF00163) Pfam (PF01479)	Escherichia coli
36	JA (e)	30S ribosomal protein S5	polymer	167	17629.4	1	UniProt (P0A7W1) Pfam (PF00333) Pfam (PF03719)	Escherichia coli
37	KA (f)	30S ribosomal protein S6	polymer	135	15727.5	1	UniProt (P02358) Pfam (PF01250)	Escherichia coli (strain K12)
38	LA (g)	30S ribosomal protein S7	polymer	179	20055.2	1	UniProt (P02359) Pfam (PF00177)	Escherichia coli (strain K12)
39	MA (h)	30S ribosomal protein S8	polymer	129	14015.4	1	UniProt (P0A7W7) Pfam (PF00410)	Escherichia coli
40	NA (i)	30S ribosomal protein S9	polymer	130	14886.3	1	UniProt (P0A7X3) Pfam (PF00380)	Escherichia coli
41	OA (j)	30S ribosomal protein S10	polymer	103	11755.6	1	UniProt (P0A7R5) Pfam (PF00338)	Escherichia coli
42	PA (k)	30S ribosomal protein S11	polymer	117	12487.2	1	UniProt (P0A7R9) Pfam (PF00411)	Escherichia coli
43	QA (l)	30S ribosomal protein S12	polymer	123	13637.0	1	UniProt (P0A7S3) Pfam (PF00164)	Escherichia coli
44	RA (m)	30S ribosomal protein S13	polymer	118	13128.5	1	UniProt (P0A7S9) Pfam (PF00416)	Escherichia coli
45	SA (n)	30S ribosomal protein S14	polymer	100	11475.4	1	UniProt (P0AG59) Pfam (PF00253)	Escherichia coli
46	TA (o)	30S ribosomal protein S15	polymer	88	10159.6	1	UniProt (P0ADZ4) Pfam (PF00312)	Escherichia coli
47	UA (p)	30S ribosomal protein S16	polymer	82	9207.6	1	UniProt (P0A7T3) Pfam (PF00886)	Escherichia coli
48	VA (q)	30S ribosomal protein S17	polymer	84	9724.5	1	UniProt (P0AG63) Pfam (PF00366)	Escherichia coli
49	WA (r)	30S ribosomal protein S18	polymer	75	9063.5	1	UniProt (P0A7T7) Pfam (PF01084)	Escherichia coli
50	XA (s)	30S ribosomal protein S19	polymer	82	9349.9	1	UniProt (P0A7U3) Pfam (PF00203)	Escherichia coli
51	YA (t)	30S ribosomal protein S20	polymer	86	9577.3	1	UniProt (P0A7U7) Pfam (PF01649)	Escherichia coli
52	ZA (u)	30S ribosomal protein S21	polymer	71	8524.0	1	UniProt (P68679) Pfam (PF01165)	Escherichia coli
53	AB (v)	Peptide chain release factor 2	polymer	383	43313.9	1	UniProt (P07012) Pfam (PF03462) Pfam (PF00472)	Escherichia coli (strain K12)	RF-2
54	BB (w)	Alternative ribosome-rescue factor A	polymer	57	6513.4	1	UniProt (P36675) Pfam (PF03889)	Escherichia coli (strain K12)
55	CB, DB (x, y)	P-site or E-site fMet-tRNA(fMet)	polymer	77	24802.8	2	GenBank (1062469497)	Escherichia coli
56	EB (z)	mRNA	polymer	18	5922.6	1		Escherichia coli
57	AC, AD, AE, AF, BC... (A, B, a)	MAGNESIUM ION	non-polymer		24.3	124	Chemie (MG)
58	FF (5)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)

Sequence modifications

A: 1 - 2904 (GenBank: 42756)

PDB	External Database	Details
A 887	U 887	conflict

a: 2 - 1534 (GenBank: 817573384)

PDB	External Database	Details
A 527	G 4164426	conflict

r: 1 - 75 (UniProt: P0A7T7)

PDB	External Database	Details
Glu 15	Ala 15	conflict

v: 1 - 365 (UniProt: P07012)

PDB	External Database	Details
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
Ser -11	-	expression tag
Ala -10	-	expression tag
Ala -9	-	expression tag
Leu -8	-	expression tag
Glu -7	-	expression tag
Val -6	-	expression tag
Leu -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
Pro -1	-	expression tag
Gly 0	-	expression tag

w: 1 - 55 (UniProt: P36675)

PDB	External Database	Details
Gly -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	57
	Total formula weight	2246225.0
Non-Polymers*	Number of molecules	125
	Total formula weight	3079.2
All*	Total formula weight	2249304.2

*Water molecules are not included.