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- EMDB-8505: Structural Basis of Co-translational Quality Control by ArfA and ... -

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Basic information

Entry
Database: EMDB / ID: EMD-8505
TitleStructural Basis of Co-translational Quality Control by ArfA and RF2 Bound to Ribosome
Map data
Sample
  • Complex: Non-stop ribosomal complex with ArfA and RF2
    • Complex: 30S subunitProkaryotic small ribosomal subunit
      • Complex: 30S ribosomal protein S2
        • Protein or peptide: x 1 types
      • Organelle or cellular component: 16S rRNA
        • RNA: x 1 types
      • Organelle or cellular component: 30S ribosomal protein S3
        • Protein or peptide: x 1 types
      • Organelle or cellular component: 30S ribosomal protein S4
        • Protein or peptide: x 1 types
      • Organelle or cellular component: 30S ribosomal protein S5
        • Protein or peptide: x 1 types
      • Organelle or cellular component: 30S ribosomal protein S6
        • Protein or peptide: x 1 types
      • Organelle or cellular component: 30S ribosomal protein S7
        • Protein or peptide: x 1 types
      • Organelle or cellular component: 30S ribosomal protein S8
        • Protein or peptide: x 1 types
      • Organelle or cellular component: 30S ribosomal protein S9
        • Protein or peptide: x 1 types
      • Organelle or cellular component: 30S ribosomal protein S10
        • Protein or peptide: x 1 types
      • Organelle or cellular component: 30S ribosomal protein S11
        • Protein or peptide: x 1 types
      • Organelle or cellular component: 30S ribosomal protein S12
        • Protein or peptide: x 1 types
      • Organelle or cellular component: 30S ribosomal protein S13
        • Protein or peptide: x 1 types
      • Organelle or cellular component: 30S ribosomal protein S14
        • Protein or peptide: x 1 types
      • Organelle or cellular component: 30S ribosomal protein S15
        • Protein or peptide: x 1 types
      • Organelle or cellular component: 30S ribosomal protein S16
        • Protein or peptide: x 1 types
      • Organelle or cellular component: 30S ribosomal protein S17
        • Protein or peptide: x 1 types
      • Organelle or cellular component: 30S ribosomal protein S18
        • Protein or peptide: x 1 types
      • Organelle or cellular component: 30S ribosomal protein S19
        • Protein or peptide: x 1 types
      • Organelle or cellular component: 30S ribosomal protein S20
        • Protein or peptide: x 1 types
      • Organelle or cellular component: 30S ribosomal protein S21
        • Protein or peptide: x 1 types
    • Complex: 50S subunitProkaryotic large ribosomal subunit
      • Complex: 50S ribosomal protein L25
        • Protein or peptide: x 1 types
      • Organelle or cellular component: 23S rRNA23S ribosomal RNA
        • RNA: x 1 types
      • Organelle or cellular component: 50S ribosomal protein L2
        • Protein or peptide: x 1 types
      • Organelle or cellular component: 50S ribosomal protein L3
        • Protein or peptide: x 1 types
      • Organelle or cellular component: 50S ribosomal protein L4
        • Protein or peptide: x 1 types
      • Organelle or cellular component: 50S ribosomal protein L5
        • Protein or peptide: x 1 types
      • Organelle or cellular component: 50S ribosomal protein L6
        • Protein or peptide: x 1 types
      • Organelle or cellular component: 50S ribosomal protein L9
        • Protein or peptide: x 1 types
      • Organelle or cellular component: 50S ribosomal protein L11
        • Protein or peptide: x 1 types
      • Organelle or cellular component: 50S ribosomal protein L13
        • Protein or peptide: x 1 types
      • Organelle or cellular component: 50S ribosomal protein L14
        • Protein or peptide: x 1 types
      • Organelle or cellular component: 50S ribosomal protein L15
        • Protein or peptide: x 1 types
      • Organelle or cellular component: 50S ribosomal protein L16
        • Protein or peptide: x 1 types
      • Organelle or cellular component: 50S ribosomal protein L17
        • Protein or peptide: x 1 types
      • Organelle or cellular component: 50S ribosomal protein L18
        • Protein or peptide: x 1 types
      • Organelle or cellular component: 50S ribosomal protein L19
        • Protein or peptide: x 1 types
      • Organelle or cellular component: 50S ribosomal protein L20
      • Organelle or cellular component: 50S ribosomal protein L21
        • Protein or peptide: x 1 types
      • Organelle or cellular component: 50S ribosomal protein L22
        • Protein or peptide: x 1 types
      • Organelle or cellular component: 50S ribosomal protein L23
        • Protein or peptide: x 1 types
      • Organelle or cellular component: 50S ribosomal protein L24
        • Protein or peptide: x 1 types
      • Organelle or cellular component: 50S ribosomal protein L27
        • Protein or peptide: x 1 types
      • Organelle or cellular component: 50S ribosomal protein L28
        • Protein or peptide: x 1 types
      • Organelle or cellular component: 50S ribosomal protein L29
        • Protein or peptide: x 1 types
      • Organelle or cellular component: 50S ribosomal protein L30
        • Protein or peptide: x 1 types
      • Organelle or cellular component: 50S ribosomal protein L32
        • Protein or peptide: x 1 types
      • Organelle or cellular component: 50S ribosomal protein L33
        • Protein or peptide: x 1 types
      • Organelle or cellular component: 50S ribosomal protein L34
        • Protein or peptide: x 1 types
      • Organelle or cellular component: 50S ribosomal protein L35
        • Protein or peptide: x 1 types
      • Organelle or cellular component: 50S ribosomal protein L36
        • Protein or peptide: x 1 types
      • Organelle or cellular component: 5S rRNA5S ribosomal RNA
        • RNA: x 1 types
      • Protein or peptide: x 1 types
    • Organelle or cellular component: Alternative ribosome-rescue factor A
      • Protein or peptide: x 1 types
    • Organelle or cellular component: Peptide chain release factor 2
      • Protein or peptide: x 1 types
    • Organelle or cellular component: P-site or E-site fMet-tRNA(fMet)
      • RNA: x 1 types
    • Organelle or cellular component: mRNAMessenger RNA
      • RNA: x 1 types
  • Ligand: x 2 types
Function / homology
Function and homology information


translation release factor activity, codon specific / stringent response / ribosomal large subunit binding / mRNA base-pairing translational repressor activity / ornithine decarboxylase inhibitor activity / misfolded RNA binding / transcription antitermination factor activity, RNA binding / Group I intron splicing / RNA folding / transcriptional attenuation ...translation release factor activity, codon specific / stringent response / ribosomal large subunit binding / mRNA base-pairing translational repressor activity / ornithine decarboxylase inhibitor activity / misfolded RNA binding / transcription antitermination factor activity, RNA binding / Group I intron splicing / RNA folding / transcriptional attenuation / endoribonuclease inhibitor activity / RNA-binding transcription regulator activity / positive regulation of ribosome biogenesis / negative regulation of cytoplasmic translation / translational termination / DnaA-L2 complex / negative regulation of translational initiation / four-way junction DNA binding / translation repressor activity / negative regulation of DNA-templated DNA replication initiation / rescue of stalled ribosome / regulation of mRNA stability / ribosome assembly / mRNA regulatory element binding translation repressor activity / response to reactive oxygen species / assembly of large subunit precursor of preribosome / transcription elongation factor complex / positive regulation of RNA splicing / cytosolic ribosome assembly / regulation of DNA-templated transcription elongation / transcription antitermination / DNA endonuclease activity / regulation of cell growth / DNA-templated transcription termination / maintenance of translational fidelity / : / response to radiation / mRNA 5'-UTR binding / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / regulation of translation / ribosome biogenesis / ribosome binding / large ribosomal subunit / 5S rRNA binding / ribosomal large subunit assembly / cytosolic large ribosomal subunit / small ribosomal subunit / cytoplasmic translation / transferase activity / tRNA binding / negative regulation of translation / molecular adaptor activity / ribosome / rRNA binding / structural constituent of ribosome / translation / response to antibiotic / mRNA binding / negative regulation of DNA-templated transcription / DNA binding / RNA binding / zinc ion binding / membrane / cytosol / cytoplasm
Similarity search - Function
Alternative ribosome-rescue factor A / Alternative ribosome-rescue factor A / Peptide chain release factor 2 / Peptide chain release factor / PCRF domain / PCRF / Peptide chain release factor class I superfamily / Prokaryotic-type class I peptide chain release factors signature. / Peptide chain release factor class I / RF-1 domain ...Alternative ribosome-rescue factor A / Alternative ribosome-rescue factor A / Peptide chain release factor 2 / Peptide chain release factor / PCRF domain / PCRF / Peptide chain release factor class I superfamily / Prokaryotic-type class I peptide chain release factors signature. / Peptide chain release factor class I / RF-1 domain / Ribosomal protein S21, conserved site / Ribosomal protein S21 signature. / Ribosomal protein L25, short-form / Ribosomal protein S14, bacterial/plastid / Ribosomal protein L11, bacterial-type / Ribosomal protein S21 superfamily / Ribosomal protein S21 / Ribosomal protein S16, conserved site / Ribosomal protein S16 signature. / Ribosomal protein S21 / Ribosomal protein L21, conserved site / Ribosomal protein L21 signature. / Ribosomal protein L11, conserved site / Ribosomal protein L11 signature. / Ribosomal protein L16 signature 1. / : / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / Ribosomal protein L16, conserved site / Ribosomal protein L16 signature 2. / Ribosomal protein L17 signature. / Ribosomal protein L9 signature. / Ribosomal protein L9, bacteria/chloroplast / Ribosomal protein L9, C-terminal / Ribosomal protein L9, C-terminal domain / Ribosomal protein L9, C-terminal domain superfamily / Ribosomal L25p family / Ribosomal protein L25 / Ribosomal protein L11, N-terminal / Ribosomal protein L11, N-terminal domain / Ribosomal protein L11/L12 / Ribosomal protein L11, C-terminal / Ribosomal protein L11, C-terminal domain superfamily / Ribosomal protein L11/L12, N-terminal domain superfamily / Ribosomal protein L11, RNA binding domain / Ribosomal protein L11/L12 / Ribosomal protein L28/L24 superfamily / Ribosomal protein L36 signature. / Ribosomal protein L25/Gln-tRNA synthetase, N-terminal / Ribosomal protein L32p, bacterial type / Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily / Ribosomal protein L9, N-terminal domain superfamily / Ribosomal protein L9 / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / Ribosomal protein L28 / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L35, non-mitochondrial / Ribosomal protein L5, bacterial-type / Ribosomal protein L6, bacterial-type / Ribosomal protein L18, bacterial-type / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein L9/RNase H1, N-terminal / Ribosomal protein L20 signature. / Ribosomal protein S3, bacterial-type / Ribosomal protein L27, conserved site / Ribosomal protein S6, conserved site / Ribosomal protein L27 signature. / Ribosomal protein S6 signature. / Ribosomal protein S19, bacterial-type / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein S11, bacterial-type / Ribosomal protein S13, bacterial-type / Ribosomal protein S20 / Ribosomal protein S20 superfamily / Ribosomal protein S20 / Ribosomal protein S9, bacterial/plastid / Ribosomal protein L14P, bacterial-type / Ribosomal protein S4, bacterial-type / Ribosomal protein L34, conserved site / Ribosomal protein L34 signature. / 30S ribosomal protein S17 / Ribosomal protein S5, bacterial-type / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein S6, plastid/chloroplast / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein L35 / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal L28 family / Ribosomal protein L33 / Ribosomal protein L33
Similarity search - Domain/homology
Small ribosomal subunit protein bS6 / Small ribosomal subunit protein uS7 / Large ribosomal subunit protein uL15 / Peptide chain release factor RF2 / Large ribosomal subunit protein uL11 / Large ribosomal subunit protein bL19 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein bL28 / Large ribosomal subunit protein uL29 ...Small ribosomal subunit protein bS6 / Small ribosomal subunit protein uS7 / Large ribosomal subunit protein uL15 / Peptide chain release factor RF2 / Large ribosomal subunit protein uL11 / Large ribosomal subunit protein bL19 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein bL28 / Large ribosomal subunit protein uL29 / Large ribosomal subunit protein bL32 / Large ribosomal subunit protein bL33 / Large ribosomal subunit protein bL34 / Large ribosomal subunit protein bL35 / Large ribosomal subunit protein bL36A / Large ribosomal subunit protein bL9 / Small ribosomal subunit protein uS10 / Small ribosomal subunit protein uS11 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein bS16 / Small ribosomal subunit protein bS18 / Small ribosomal subunit protein uS19 / Small ribosomal subunit protein bS20 / Small ribosomal subunit protein uS2 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS5 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein uS9 / Large ribosomal subunit protein uL13 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein uL23 / Small ribosomal subunit protein uS15 / Large ribosomal subunit protein bL17 / Large ribosomal subunit protein bL21 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein uL6 / Small ribosomal subunit protein uS14 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL18 / Alternative ribosome-rescue factor A / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein uL5 / Small ribosomal subunit protein bS21 / Large ribosomal subunit protein bL25
Similarity search - Component
Biological speciesEscherichia coli (E. coli) / Escherichia coli (strain K12) (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.5 Å
AuthorsZeng F / Chen Y / Remis J / Shekhar M / Phillips JC / Tajkhorshid E / Jin H
Funding support United States, 1 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R01GM120552 United States
CitationJournal: Nature / Year: 2017
Title: Structural basis of co-translational quality control by ArfA and RF2 bound to ribosome.
Authors: Fuxing Zeng / Yanbo Chen / Jonathan Remis / Mrinal Shekhar / James C Phillips / Emad Tajkhorshid / Hong Jin /
Abstract: Quality control mechanisms intervene appropriately when defective translation events occur, in order to preserve the integrity of protein synthesis. Rescue of ribosomes translating on messenger RNAs ...Quality control mechanisms intervene appropriately when defective translation events occur, in order to preserve the integrity of protein synthesis. Rescue of ribosomes translating on messenger RNAs that lack stop codons is one of the co-translational quality control pathways. In many bacteria, ArfA recognizes stalled ribosomes and recruits the release factor RF2, which catalyses the termination of protein synthesis. Although an induced-fit mechanism of nonstop mRNA surveillance mediated by ArfA and RF2 has been reported, the molecular interaction between ArfA and RF2 in the ribosome that is responsible for the mechanism is unknown. Here we report an electron cryo-microscopy structure of ArfA and RF2 in complex with the 70S ribosome bound to a nonstop mRNA. The structure, which is consistent with our kinetic and biochemical data, reveals the molecular interactions that enable ArfA to specifically recruit RF2, not RF1, into the ribosome and to enable RF2 to release the truncated protein product in this co-translational quality control pathway. The positively charged C-terminal domain of ArfA anchors in the mRNA entry channel of the ribosome. Furthermore, binding of ArfA and RF2 induces conformational changes in the ribosomal decoding centre that are similar to those seen in other protein-involved decoding processes. Specific interactions between residues in the N-terminal domain of ArfA and RF2 help RF2 to adopt a catalytically competent conformation for peptide release. Our findings provide a framework for understanding recognition of the translational state of the ribosome by new proteins, and expand our knowledge of the decoding potential of the ribosome.
History
DepositionDec 4, 2016-
Header (metadata) releaseJan 4, 2017-
Map releaseJan 11, 2017-
UpdateDec 2, 2020-
Current statusDec 2, 2020Processing site: RCSB / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.017
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by height
  • Surface level: 0.017
  • Imaged by UCSF Chimera
  • Download
  • Surface view with fitted model
  • Atomic models: PDB-5u4i
  • Surface level: 0.017
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

Downloads & links

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Map

FileDownload / File: emd_8505.map.gz / Format: CCP4 / Size: 216 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 1.193 Å
Density
Contour LevelBy AUTHOR: 0.02 / Movie #1: 0.017
Minimum - Maximum-0.061812814 - 0.11835373
Average (Standard dev.)0.00000706809 (±0.0042961156)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions384384384
Spacing384384384
CellA=B=C: 458.112 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.1931.1931.193
M x/y/z384384384
origin x/y/z0.0000.0000.000
length x/y/z458.112458.112458.112
α/β/γ90.00090.00090.000
start NX/NY/NZ000
NX/NY/NZ256256256
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS384384384
D min/max/mean-0.0620.1180.000

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Supplemental data

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Half map: #1

Fileemd_8505_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

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Half map: #2

Fileemd_8505_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

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Sample components

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Entire : Non-stop ribosomal complex with ArfA and RF2

EntireName: Non-stop ribosomal complex with ArfA and RF2
Components
  • Complex: Non-stop ribosomal complex with ArfA and RF2
    • Complex: 30S subunitProkaryotic small ribosomal subunit
      • Complex: 30S ribosomal protein S2
        • Protein or peptide: 30S ribosomal protein S2
      • Organelle or cellular component: 16S rRNA
        • RNA: 16S rRNA
      • Organelle or cellular component: 30S ribosomal protein S3
        • Protein or peptide: 30S ribosomal protein S3
      • Organelle or cellular component: 30S ribosomal protein S4
        • Protein or peptide: 30S ribosomal protein S4
      • Organelle or cellular component: 30S ribosomal protein S5
        • Protein or peptide: 30S ribosomal protein S5
      • Organelle or cellular component: 30S ribosomal protein S6
        • Protein or peptide: 30S ribosomal protein S6
      • Organelle or cellular component: 30S ribosomal protein S7
        • Protein or peptide: 30S ribosomal protein S7
      • Organelle or cellular component: 30S ribosomal protein S8
        • Protein or peptide: 30S ribosomal protein S8
      • Organelle or cellular component: 30S ribosomal protein S9
        • Protein or peptide: 30S ribosomal protein S9
      • Organelle or cellular component: 30S ribosomal protein S10
        • Protein or peptide: 30S ribosomal protein S10
      • Organelle or cellular component: 30S ribosomal protein S11
        • Protein or peptide: 30S ribosomal protein S11
      • Organelle or cellular component: 30S ribosomal protein S12
        • Protein or peptide: 30S ribosomal protein S12
      • Organelle or cellular component: 30S ribosomal protein S13
        • Protein or peptide: 30S ribosomal protein S13
      • Organelle or cellular component: 30S ribosomal protein S14
        • Protein or peptide: 30S ribosomal protein S14
      • Organelle or cellular component: 30S ribosomal protein S15
        • Protein or peptide: 30S ribosomal protein S15
      • Organelle or cellular component: 30S ribosomal protein S16
        • Protein or peptide: 30S ribosomal protein S16
      • Organelle or cellular component: 30S ribosomal protein S17
        • Protein or peptide: 30S ribosomal protein S17
      • Organelle or cellular component: 30S ribosomal protein S18
        • Protein or peptide: 30S ribosomal protein S18
      • Organelle or cellular component: 30S ribosomal protein S19
        • Protein or peptide: 30S ribosomal protein S19
      • Organelle or cellular component: 30S ribosomal protein S20
        • Protein or peptide: 30S ribosomal protein S20
      • Organelle or cellular component: 30S ribosomal protein S21
        • Protein or peptide: 30S ribosomal protein S21
    • Complex: 50S subunitProkaryotic large ribosomal subunit
      • Complex: 50S ribosomal protein L25
        • Protein or peptide: 50S ribosomal protein L25
      • Organelle or cellular component: 23S rRNA23S ribosomal RNA
        • RNA: 23S rRNA23S ribosomal RNA
      • Organelle or cellular component: 50S ribosomal protein L2
        • Protein or peptide: 50S ribosomal protein L2
      • Organelle or cellular component: 50S ribosomal protein L3
        • Protein or peptide: 50S ribosomal protein L3
      • Organelle or cellular component: 50S ribosomal protein L4
        • Protein or peptide: 50S ribosomal protein L4
      • Organelle or cellular component: 50S ribosomal protein L5
        • Protein or peptide: 50S ribosomal protein L5
      • Organelle or cellular component: 50S ribosomal protein L6
        • Protein or peptide: 50S ribosomal protein L6
      • Organelle or cellular component: 50S ribosomal protein L9
        • Protein or peptide: 50S ribosomal protein L9
      • Organelle or cellular component: 50S ribosomal protein L11
        • Protein or peptide: 50S ribosomal protein L11
      • Organelle or cellular component: 50S ribosomal protein L13
        • Protein or peptide: 50S ribosomal protein L13
      • Organelle or cellular component: 50S ribosomal protein L14
        • Protein or peptide: 50S ribosomal protein L14
      • Organelle or cellular component: 50S ribosomal protein L15
        • Protein or peptide: 50S ribosomal protein L15
      • Organelle or cellular component: 50S ribosomal protein L16
        • Protein or peptide: 50S ribosomal protein L16
      • Organelle or cellular component: 50S ribosomal protein L17
        • Protein or peptide: 50S ribosomal protein L17
      • Organelle or cellular component: 50S ribosomal protein L18
        • Protein or peptide: 50S ribosomal protein L18
      • Organelle or cellular component: 50S ribosomal protein L19
        • Protein or peptide: 50S ribosomal protein L19
      • Organelle or cellular component: 50S ribosomal protein L20
      • Organelle or cellular component: 50S ribosomal protein L21
        • Protein or peptide: 50S ribosomal protein L21
      • Organelle or cellular component: 50S ribosomal protein L22
        • Protein or peptide: 50S ribosomal protein L22
      • Organelle or cellular component: 50S ribosomal protein L23
        • Protein or peptide: 50S ribosomal protein L23
      • Organelle or cellular component: 50S ribosomal protein L24
        • Protein or peptide: 50S ribosomal protein L24
      • Organelle or cellular component: 50S ribosomal protein L27
        • Protein or peptide: 50S ribosomal protein L27
      • Organelle or cellular component: 50S ribosomal protein L28
        • Protein or peptide: 50S ribosomal protein L28
      • Organelle or cellular component: 50S ribosomal protein L29
        • Protein or peptide: 50S ribosomal protein L29
      • Organelle or cellular component: 50S ribosomal protein L30
        • Protein or peptide: 50S ribosomal protein L30
      • Organelle or cellular component: 50S ribosomal protein L32
        • Protein or peptide: 50S ribosomal protein L32
      • Organelle or cellular component: 50S ribosomal protein L33
        • Protein or peptide: 50S ribosomal protein L33
      • Organelle or cellular component: 50S ribosomal protein L34
        • Protein or peptide: 50S ribosomal protein L34
      • Organelle or cellular component: 50S ribosomal protein L35
        • Protein or peptide: 50S ribosomal protein L35
      • Organelle or cellular component: 50S ribosomal protein L36
        • Protein or peptide: 50S ribosomal protein L36
      • Organelle or cellular component: 5S rRNA5S ribosomal RNA
        • RNA: 5S rRNA5S ribosomal RNA
      • Protein or peptide: 50S ribosomal protein L20
    • Organelle or cellular component: Alternative ribosome-rescue factor A
      • Protein or peptide: Alternative ribosome-rescue factor A
    • Organelle or cellular component: Peptide chain release factor 2
      • Protein or peptide: Peptide chain release factor 2
    • Organelle or cellular component: P-site or E-site fMet-tRNA(fMet)
      • RNA: P-site or E-site fMet-tRNA(fMet)
    • Organelle or cellular component: mRNAMessenger RNA
      • RNA: mRNAMessenger RNA
  • Ligand: MAGNESIUM ION
  • Ligand: ZINC ION

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Supramolecule #1: Non-stop ribosomal complex with ArfA and RF2

SupramoleculeName: Non-stop ribosomal complex with ArfA and RF2 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#56
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #2: 30S subunit

SupramoleculeName: 30S subunit / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #32-#52

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Supramolecule #3: 50S subunit

SupramoleculeName: 50S subunit / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #1-#31

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Supramolecule #11: 30S ribosomal protein S2

SupramoleculeName: 30S ribosomal protein S2 / type: complex / ID: 11 / Parent: 2 / Macromolecule list: #33
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #49: 50S ribosomal protein L25

SupramoleculeName: 50S ribosomal protein L25 / type: complex / ID: 49 / Parent: 3 / Macromolecule list: #22
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #4: Alternative ribosome-rescue factor A

SupramoleculeName: Alternative ribosome-rescue factor A / type: organelle_or_cellular_component / ID: 4 / Parent: 1 / Macromolecule list: #54
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 6.35 kDa/nm
Recombinant expressionOrganism: Escherichia coli (E. coli) / Recombinant strain: BL21 / Recombinant plasmid: pET28

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Supramolecule #5: Peptide chain release factor 2

SupramoleculeName: Peptide chain release factor 2 / type: organelle_or_cellular_component / ID: 5 / Parent: 1 / Macromolecule list: #53
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 40.13 kDa/nm
Recombinant expressionOrganism: Escherichia coli (E. coli) / Recombinant strain: BL21 / Recombinant plasmid: pETNKI-his3C-LIC-Kan

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Supramolecule #6: P-site or E-site fMet-tRNA(fMet)

SupramoleculeName: P-site or E-site fMet-tRNA(fMet) / type: organelle_or_cellular_component / ID: 6 / Parent: 1 / Macromolecule list: #55 / Details: fMet tRNA was in vitro transcribed

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Supramolecule #7: mRNA

SupramoleculeName: mRNA / type: organelle_or_cellular_component / ID: 7 / Parent: 1 / Macromolecule list: #56

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Supramolecule #8: 23S rRNA

SupramoleculeName: 23S rRNA / type: organelle_or_cellular_component / ID: 8 / Parent: 3 / Macromolecule list: #1
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #9: 50S ribosomal protein L2

SupramoleculeName: 50S ribosomal protein L2 / type: organelle_or_cellular_component / ID: 9 / Parent: 3 / Macromolecule list: #3
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #10: 16S rRNA

SupramoleculeName: 16S rRNA / type: organelle_or_cellular_component / ID: 10 / Parent: 2 / Macromolecule list: #32
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #12: 30S ribosomal protein S3

SupramoleculeName: 30S ribosomal protein S3 / type: organelle_or_cellular_component / ID: 12 / Parent: 2 / Macromolecule list: #34
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #13: 30S ribosomal protein S4

SupramoleculeName: 30S ribosomal protein S4 / type: organelle_or_cellular_component / ID: 13 / Parent: 2 / Macromolecule list: #35
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #14: 30S ribosomal protein S5

SupramoleculeName: 30S ribosomal protein S5 / type: organelle_or_cellular_component / ID: 14 / Parent: 2 / Macromolecule list: #36
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #15: 30S ribosomal protein S6

SupramoleculeName: 30S ribosomal protein S6 / type: organelle_or_cellular_component / ID: 15 / Parent: 2 / Macromolecule list: #37
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #16: 30S ribosomal protein S7

SupramoleculeName: 30S ribosomal protein S7 / type: organelle_or_cellular_component / ID: 16 / Parent: 2 / Macromolecule list: #38
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #17: 30S ribosomal protein S8

SupramoleculeName: 30S ribosomal protein S8 / type: organelle_or_cellular_component / ID: 17 / Parent: 2 / Macromolecule list: #39
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #18: 30S ribosomal protein S9

SupramoleculeName: 30S ribosomal protein S9 / type: organelle_or_cellular_component / ID: 18 / Parent: 2 / Macromolecule list: #40
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #19: 30S ribosomal protein S10

SupramoleculeName: 30S ribosomal protein S10 / type: organelle_or_cellular_component / ID: 19 / Parent: 2 / Macromolecule list: #41
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #20: 30S ribosomal protein S11

SupramoleculeName: 30S ribosomal protein S11 / type: organelle_or_cellular_component / ID: 20 / Parent: 2 / Macromolecule list: #42
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #21: 30S ribosomal protein S12

SupramoleculeName: 30S ribosomal protein S12 / type: organelle_or_cellular_component / ID: 21 / Parent: 2 / Macromolecule list: #43
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #22: 30S ribosomal protein S13

SupramoleculeName: 30S ribosomal protein S13 / type: organelle_or_cellular_component / ID: 22 / Parent: 2 / Macromolecule list: #44
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #23: 30S ribosomal protein S14

SupramoleculeName: 30S ribosomal protein S14 / type: organelle_or_cellular_component / ID: 23 / Parent: 2 / Macromolecule list: #45
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #24: 30S ribosomal protein S15

SupramoleculeName: 30S ribosomal protein S15 / type: organelle_or_cellular_component / ID: 24 / Parent: 2 / Macromolecule list: #46
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #25: 30S ribosomal protein S16

SupramoleculeName: 30S ribosomal protein S16 / type: organelle_or_cellular_component / ID: 25 / Parent: 2 / Macromolecule list: #47
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #26: 30S ribosomal protein S17

SupramoleculeName: 30S ribosomal protein S17 / type: organelle_or_cellular_component / ID: 26 / Parent: 2 / Macromolecule list: #48
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #27: 30S ribosomal protein S18

SupramoleculeName: 30S ribosomal protein S18 / type: organelle_or_cellular_component / ID: 27 / Parent: 2 / Macromolecule list: #49
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #28: 30S ribosomal protein S19

SupramoleculeName: 30S ribosomal protein S19 / type: organelle_or_cellular_component / ID: 28 / Parent: 2 / Macromolecule list: #50
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #29: 30S ribosomal protein S20

SupramoleculeName: 30S ribosomal protein S20 / type: organelle_or_cellular_component / ID: 29 / Parent: 2 / Macromolecule list: #51
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #30: 30S ribosomal protein S21

SupramoleculeName: 30S ribosomal protein S21 / type: organelle_or_cellular_component / ID: 30 / Parent: 2 / Macromolecule list: #52
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #31: 50S ribosomal protein L3

SupramoleculeName: 50S ribosomal protein L3 / type: organelle_or_cellular_component / ID: 31 / Parent: 3 / Macromolecule list: #4
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #32: 50S ribosomal protein L4

SupramoleculeName: 50S ribosomal protein L4 / type: organelle_or_cellular_component / ID: 32 / Parent: 3 / Macromolecule list: #5
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #33: 50S ribosomal protein L5

SupramoleculeName: 50S ribosomal protein L5 / type: organelle_or_cellular_component / ID: 33 / Parent: 3 / Macromolecule list: #6
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #34: 50S ribosomal protein L6

SupramoleculeName: 50S ribosomal protein L6 / type: organelle_or_cellular_component / ID: 34 / Parent: 3 / Macromolecule list: #7
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #35: 50S ribosomal protein L9

SupramoleculeName: 50S ribosomal protein L9 / type: organelle_or_cellular_component / ID: 35 / Parent: 3 / Macromolecule list: #8
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #36: 50S ribosomal protein L11

SupramoleculeName: 50S ribosomal protein L11 / type: organelle_or_cellular_component / ID: 36 / Parent: 3 / Macromolecule list: #9
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #37: 50S ribosomal protein L13

SupramoleculeName: 50S ribosomal protein L13 / type: organelle_or_cellular_component / ID: 37 / Parent: 3 / Macromolecule list: #10
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #38: 50S ribosomal protein L14

SupramoleculeName: 50S ribosomal protein L14 / type: organelle_or_cellular_component / ID: 38 / Parent: 3 / Macromolecule list: #11
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #39: 50S ribosomal protein L15

SupramoleculeName: 50S ribosomal protein L15 / type: organelle_or_cellular_component / ID: 39 / Parent: 3 / Macromolecule list: #12
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #40: 50S ribosomal protein L16

SupramoleculeName: 50S ribosomal protein L16 / type: organelle_or_cellular_component / ID: 40 / Parent: 3 / Macromolecule list: #13
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #41: 50S ribosomal protein L17

SupramoleculeName: 50S ribosomal protein L17 / type: organelle_or_cellular_component / ID: 41 / Parent: 3 / Macromolecule list: #14
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #42: 50S ribosomal protein L18

SupramoleculeName: 50S ribosomal protein L18 / type: organelle_or_cellular_component / ID: 42 / Parent: 3 / Macromolecule list: #15
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #43: 50S ribosomal protein L19

SupramoleculeName: 50S ribosomal protein L19 / type: organelle_or_cellular_component / ID: 43 / Parent: 3 / Macromolecule list: #16
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #44: 50S ribosomal protein L20

SupramoleculeName: 50S ribosomal protein L20 / type: organelle_or_cellular_component / ID: 44 / Parent: 3
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #45: 50S ribosomal protein L21

SupramoleculeName: 50S ribosomal protein L21 / type: organelle_or_cellular_component / ID: 45 / Parent: 3 / Macromolecule list: #18
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #46: 50S ribosomal protein L22

SupramoleculeName: 50S ribosomal protein L22 / type: organelle_or_cellular_component / ID: 46 / Parent: 3 / Macromolecule list: #19
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #47: 50S ribosomal protein L23

SupramoleculeName: 50S ribosomal protein L23 / type: organelle_or_cellular_component / ID: 47 / Parent: 3 / Macromolecule list: #20
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #48: 50S ribosomal protein L24

SupramoleculeName: 50S ribosomal protein L24 / type: organelle_or_cellular_component / ID: 48 / Parent: 3 / Macromolecule list: #21
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #50: 50S ribosomal protein L27

SupramoleculeName: 50S ribosomal protein L27 / type: organelle_or_cellular_component / ID: 50 / Parent: 3 / Macromolecule list: #23
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #51: 50S ribosomal protein L28

SupramoleculeName: 50S ribosomal protein L28 / type: organelle_or_cellular_component / ID: 51 / Parent: 3 / Macromolecule list: #24
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #52: 50S ribosomal protein L29

SupramoleculeName: 50S ribosomal protein L29 / type: organelle_or_cellular_component / ID: 52 / Parent: 3 / Macromolecule list: #25
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #53: 50S ribosomal protein L30

SupramoleculeName: 50S ribosomal protein L30 / type: organelle_or_cellular_component / ID: 53 / Parent: 3 / Macromolecule list: #26
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #54: 50S ribosomal protein L32

SupramoleculeName: 50S ribosomal protein L32 / type: organelle_or_cellular_component / ID: 54 / Parent: 3 / Macromolecule list: #27
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #55: 50S ribosomal protein L33

SupramoleculeName: 50S ribosomal protein L33 / type: organelle_or_cellular_component / ID: 55 / Parent: 3 / Macromolecule list: #28
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #56: 50S ribosomal protein L34

SupramoleculeName: 50S ribosomal protein L34 / type: organelle_or_cellular_component / ID: 56 / Parent: 3 / Macromolecule list: #29
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #57: 50S ribosomal protein L35

SupramoleculeName: 50S ribosomal protein L35 / type: organelle_or_cellular_component / ID: 57 / Parent: 3 / Macromolecule list: #30
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #58: 50S ribosomal protein L36

SupramoleculeName: 50S ribosomal protein L36 / type: organelle_or_cellular_component / ID: 58 / Parent: 3 / Macromolecule list: #31
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #59: 5S rRNA

SupramoleculeName: 5S rRNA / type: organelle_or_cellular_component / ID: 59 / Parent: 3 / Macromolecule list: #2
Source (natural)Organism: Escherichia coli (E. coli)

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Macromolecule #1: 23S rRNA

MacromoleculeName: 23S rRNA / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 941.761625 KDa
SequenceString: GGUUAAGCGA CUAAGCGUAC ACGGUGGAUG CCCUGGCAGU CAGAGGCGAU GAAGGACGUG CUAAUCUGCG AUAAGCGUCG GUAAGGUGA UAUGAACCGU UAUAACCGGC GAUUUCCGAA UGGGGAAACC CAGUGUGUUU CGACACACUA UCAUUAACUG A AUCCAUAG ...String:
GGUUAAGCGA CUAAGCGUAC ACGGUGGAUG CCCUGGCAGU CAGAGGCGAU GAAGGACGUG CUAAUCUGCG AUAAGCGUCG GUAAGGUGA UAUGAACCGU UAUAACCGGC GAUUUCCGAA UGGGGAAACC CAGUGUGUUU CGACACACUA UCAUUAACUG A AUCCAUAG GUUAAUGAGG CGAACCGGGG GAACUGAAAC AUCUAAGUAC CCCGAGGAAA AGAAAUCAAC CGAGAUUCCC CC AGUAGCG GCGAGCGAAC GGGGAGCAGC CCAGAGCCUG AAUCAGUGUG UGUGUUAGUG GAAGCGUCUG GAAAGGCGCG CGA UACAGG GUGACAGCCC CGUACACAAA AAUGCACAUG CUGUGAGCUC GAUGAGUAGG GCGGGACACG UGGUAUCCUG UCUG AAUAU GGGGGGACCA UCCUCCAAGG CUAAAUACUC CUGACUGACC GAUAGUGAAC CAGUACCGUG AGGGAAAGGC GAAAA GAAC CCCGGCGAGG GGAGUGAAAA AGAACCUGAA ACCGUGUACG UACAAGCAGU GGGAGCACGC UUAGGCGUGU GACUGC GUA CCUUUUGUAU AAUGGGUCAG CGACUUAUAU UCUGUAGCAA GGUUAACCGA AUAGGGGAGC CGAAGGGAAA CCGAGUC UU AACUGGGCGU UAAGUUGCAG GGUAUAGACC CGAAACCCGG UGAUCUAGCC AUGGGCAGGU UGAAGGUUGG GUAACACU A ACUGGAGGAC CGAACCGACU AAU(1MG)(PSU)(5MU)GAAA AAUUAGCGGA UGACUUGUGG CUGGGGGUGA AAGGCCA AU CAAACCGGGA GAUAGCUGGU UCUCCCCGAA AGCUAUUUAG GUAGCGCCUC GUGAAUUCAU CUCCGGGGGU AGAGCACU G UUUCGGCAAG GGGGUCAACC CGACUUACCA ACCCGAUGCA AACUGCGAAU ACCGGAGAAU GUUAUCACGG GAGACACAC GGCGGG(PSU)GCU AACGUCCGUC GUGAAGAGGG AAACAACCCA GACCGCCAGC UAAGGUCCCA AAGUCAUGGU UAAGUG GGA AACGAUGUGG GAAGGCCCAG ACAGCCAGGA UGUUGGCUUA GAAGCAGCCA UCAUUUAAAG AAAGCGUAAU AGCUCAC UG GUCGAGUCGG CCUGCGCGGA AGAUGUAACG GGGCUAAACC AUGCACCGAA GCUGCGGCAG CGACGCUUAU GCGUUGUU G GGUAGGGGAG CGUUCUGUAA GCCUGCGAAG GUGUGCUGUG AGGCAUGCUG GAGGUAUCAG AAGUGCGAAU GCUGACAUA AGUAACGAUA AAGCGGGUGA AAAGCCCGCU CGCCGGAAGA CCAAGGGUUC CUGUCCAACG UUAAUCGGGG CAGGGUGAGU CGACCCCUA AGGCGAGGCC GAAAGGCGUA GUCGAUGGGA AACAGGUUAA UAUUCCUGUA CUUGGUGUUA CUGCGAAGGG G GGACGGAG AAGGCUAUGU UGGCCGGGCG ACGGUUGUCC CGGUUUAAGC GUGUAGGCUG GUUUUCCAGG CAAAUCCGGA AA AUCAAGG CUGAGGCGUG AUGACGAGGC ACUACGGUGC UGAAGCAACA AAUGCCCUGC UUCCAGGAAA AGCCUCUAAG CAU CAGGUA ACAUCAAAUC GUACCCCAAA CCGACACAGG UGGUCAGGUA GAGAAUACCA AGGCGCUUGA GAGAACUCGG GUGA AGGAA CUAGGCAAAA UGGUGCCGUA ACUUCGGGAG AAGGCACGCU GAUAUGUAGG UGAGGUCCCU CGCGGAUGGA GCUGA AAUC AGUCGAAGAU ACCAGCUGGC UGCAACUGUU UAUUAAAAAC ACAGCACUGU GCAAACACGA AAGUGGACGU AUACGG UGU GACGCCU(2MG)CC CGGUGCCGGA AGGUUAAUUG AUGGGGUUAG CGCAAGCGAA GCUCUUGAUC GAAGCCCCGG UAA ACGGCG GCCG(PSU)AACUA (PSU)AACGGUCCU AAGGUAGCGA AA(5MU)UCCUUGU CGGGUAAGUU CCGAC(5MC)UG C ACGAAUGGCG UAAUGAUGGC CAGGCUGUCU CCACCCGAGA CUCAGUGAAA UUGAACUCGC UGUGAAGAUG CAGUGUACC CGCGGCAAGA CGGAAAGACC CCGUGAACCU UUACUAUAGC UUGACACUGA ACAUUGAGCC UUGAUGUGUA GGAUAGGUGG GAGGCUUUG AAGUGUGGAC GCCAGUCUGC AUGGAGCCGA CCUUGAAAUA CCACCCUUUA AUGUUUGAUG UUCUAACGUU G ACCCGUAA UCCGGGUUGC GGACAGUGUC UGGUGGGUAG UUUGACUG(OMG)G GCGGUCUCCU CCUAAAGAGU AACGGAGG A GCACGAAGGU UGGCUAAUCC UGGUCGGACA UCAGGAGGUU AGUGCAAUGG CAUAAGCCAG CUUGACUGCG AGCGUGACG GCGCGAGCAG GUGCGAAAGC AGGUCAUAGU GAUCCGGUGG UUCUGAAUGG AAGGGCCAUC GCUCAACGGA UAAAAGGUAC UCCG(2MG)GGAU AACAGGC(PSU)GA UACCGCCCAA GAGUUCAUAU CGACGGCGGU GUUUGGCA(OMC)C UCGA(PSU) GUCG GCUCAUCACA UCCUGGGGCU GAAGUAGGUC CCAAGGGUAU GGC(OMU)GUUCGC CAUUUAAAGU GGUACGCGAG C (PSU)GGGUUUA GAACGUCGUG AGACAGU(PSU)CG GUCCCUAUCU GCCGUGGGCG CUGGAGAACU GAGGGGGGCU GCUC CUAGU ACGAGAGGAC CGGAGUGGAC GCAUCACUGG UGUUCGGGUU GUCAUGCCAA UGGCACUGCC CGGUAGCUAA AUGCG GAAG AGAUAAGUGC UGAAAGCAUC UAAGCACGAA ACUUGCCCCG AGAUGAGUUC UCCCUGACCC UUUAAGGGUC CUGAAG GAA CGUUGAAGAC GACGACGUUG AUAGGCCGGG UGUGUAAGCG CAGCGAUGCG UUGAGCUAAC CGGUACUAAU GAACCGU GA GGCUUAACCU U

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Macromolecule #2: 5S rRNA

MacromoleculeName: 5S rRNA / type: rna / ID: 2 / Number of copies: 1
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 38.177762 KDa
SequenceString:
GCCUGGCGGC CGUAGCGCGG UGGUCCCACC UGACCCCAUG CCGAACUCAG AAGUGAAACG CCGUAGCGCC GAUGGUAGUG UGGGGUCUC CCCAUGCGAG AGUAGGGAAC UGCCAGGCA

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Macromolecule #32: 16S rRNA

MacromoleculeName: 16S rRNA / type: rna / ID: 32 / Number of copies: 1
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 497.04475 KDa
SequenceString: AAUUGAAGAG UUUGAUCAUG GCUCAGAUUG AACGCUGGCG GCAGGCCUAA CACAUGCAAG UCGAACGGUA ACAGGAAGAA GCUUGCUUC UUUGCUGACG AGUGGCGGAC GGGUGAGUAA UGUCUGGGAA ACUGCCUGAU GGAGGGGGAU AACUACUGGA A ACGGUAGC ...String:
AAUUGAAGAG UUUGAUCAUG GCUCAGAUUG AACGCUGGCG GCAGGCCUAA CACAUGCAAG UCGAACGGUA ACAGGAAGAA GCUUGCUUC UUUGCUGACG AGUGGCGGAC GGGUGAGUAA UGUCUGGGAA ACUGCCUGAU GGAGGGGGAU AACUACUGGA A ACGGUAGC UAAUACCGCA UAACGUCGCA AGACCAAAGA GGGGGACCUU CGGGCCUCUU GCCAUCGGAU GUGCCCAGAU GG GAUUAGC UAGUAGGUGG GGUAACGGCU CACCUAGGCG ACGAUCCCUA GCUGGUCUGA GAGGAUGACC AGCCACACUG GAA CUGAGA CACGGUCCAG ACUCCUACGG GAGGCAGCAG UGGGGAAUAU UGCACAAUGG GCGCAAGCCU GAUGCAGCCA UGCC GCGUG UAUGAAGAAG GCCUUCGGGU UGUAAAGUAC UUUCAGCGGG GAGGAAGGGA GUAAAGUUAA UACCUUUGCU CAUUG ACGU UACCCGCAGA AGAAGCACCG GCUAACUCCG (PSU)GCCAGCAGC CACGGUAAUA CGGAGGGUGC AAGCGUUAAU CG GAAUUAC UGGGCGUAAA GCGCACGCAG GCGGUUUGUU AAGUCAGAUG UGAAAUCCCC GGGCUCAACC UGGGAACUGC AUC UGAUAC UGGCAAGCUU GAGUCUCGUA GAGGGGGGUA GAAUUCCAGG UGUAGCGGUG AAAUGCGUAG AGAUCUGGAG GAAU ACCGG UGGCGAAGGC GGCCCCCUGG ACGAAGACUG ACGCUCAGGU GCGAAAGCGU GGGGAGCAAA CAGGAUUAGA UACCC UGGU AGUCCACGCC GUAAACGAUG UCGACUUGGA GGUUGUGCCC UUGAGGCGUG GCUUCCGGAG CUAACGCGUU AAGUCG ACC GCCUGGGGAG UACGGCCGCA AGGUUAAAAC UCAAAUGAAU UGACGGGGGC CCGCACAAGC GGUGGAGCAU GUGGUUU AA UUCGAU(2MG)(5MC)AA CGCGAAGAAC CUUACCUGGU CUUGACAUCC ACGGAAGUUU UCAGAGAUGA GAAUGUGCCU UCGGGAACC GUGAGACAGG UGCUGCAUGG CUGUCGUCAG CUCGUGUUGU GAAAUGUUGG GUUAAGUCCC GCAACGAGCG C AACCCUUA UCCUUUGUUG CCAGCGGUCC GGCCGGGAAC UCAAAGGAGA CUGCCAGUGA UAAACUGGAG GAAGGUGGGG AU GACGUCA AGUCAUCAUG (2MG)CCCUUACGA CCAGGGCUAC ACACGUGCUA CAAUGGCGCA UACAAAGAGA AGCGACCUC GCGAGAGCAA GCGGACCUCA UAAAGUGCGU CGUAGUCCGG AUUGGAGUCU GCAACUCGAC UCCAUGAAGU CGGAAUCGCU AGUAAUCGU GGAUCAGAAU GCCACGGUGA AUACGUUCCC GGGCCUUGUA CACACCG(4OC)CC GU(5MC)ACACCAU GGG AGUGGG UUGCAAAAGA AGUAGGUAGC UUAACCUUCG GGAGGGCGCU UACCACUUUG UGAUUCAUGA CUGGGGUGAA GUCG (UR3)AACA AGGUAACCGU AGG(2MG)G(MA6)(MA6)CCU GCGGUUGGAU CA

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Macromolecule #55: P-site or E-site fMet-tRNA(fMet)

MacromoleculeName: P-site or E-site fMet-tRNA(fMet) / type: rna / ID: 55 / Number of copies: 2
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 24.802785 KDa
SequenceString:
CGCGGGGUGG AGCAGCCUGG UAGCUCGUCG GGCUCAUAAC CCGAAGGUCG UCGGUUCAAA UCCGGCCCCC GCAACCA

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Macromolecule #56: mRNA

MacromoleculeName: mRNA / type: rna / ID: 56 / Number of copies: 1
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 5.922644 KDa
SequenceString:
GGCAAGGAGG UAAAAAUG

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Macromolecule #3: 50S ribosomal protein L2

MacromoleculeName: 50S ribosomal protein L2 / type: protein_or_peptide / ID: 3 / Details: Gene Name(s): rplB b3317 JW3279 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (strain K12) (bacteria) / Strain: K12
Molecular weightTheoretical: 29.923619 KDa
SequenceString: MAVVKCKPTS PGRRHVVKVV NPELHKGKPF APLLEKNSKS GGRNNNGRIT TRHIGGGHKQ AYRIVDFKRN KDGIPAVVER LEYDPNRSA NIALVLYKDG ERRYILAPKG LKAGDQIQSG VDAAIKPGNT LPMRNIPVGS TVHNVEMKPG KGGQLARSAG T YVQIVARD ...String:
MAVVKCKPTS PGRRHVVKVV NPELHKGKPF APLLEKNSKS GGRNNNGRIT TRHIGGGHKQ AYRIVDFKRN KDGIPAVVER LEYDPNRSA NIALVLYKDG ERRYILAPKG LKAGDQIQSG VDAAIKPGNT LPMRNIPVGS TVHNVEMKPG KGGQLARSAG T YVQIVARD GAYVTLRLRS GEMRKVEADC RATLGEVGNA EHMLRVLGKA GAARWRGVRP TVRGTAMNPV DHPHGGGEGR NF GKHPVTP WGVQTKGKKT RSNKRTDKFI VRRRSK

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Macromolecule #4: 50S ribosomal protein L3

MacromoleculeName: 50S ribosomal protein L3 / type: protein_or_peptide / ID: 4 / Details: Gene Name(s): rplC b3320 JW3282 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (strain K12) (bacteria) / Strain: K12
Molecular weightTheoretical: 22.277535 KDa
SequenceString: MIGLVGKKVG MTRIFTEDGV SIPVTVIEVE ANRVTQVKDL ANDGYRAIQV TTGAKKANRV TKPEAGHFAK AGVEAGRGLW EFRLAEGEE FTVGQSISVE LFADVKKVDV TGTSKGKGFA GTVKRWNFRT QDATHGNSLS HRVPGSIGQN QTPGKVFKGK K MAGQMGNE ...String:
MIGLVGKKVG MTRIFTEDGV SIPVTVIEVE ANRVTQVKDL ANDGYRAIQV TTGAKKANRV TKPEAGHFAK AGVEAGRGLW EFRLAEGEE FTVGQSISVE LFADVKKVDV TGTSKGKGFA GTVKRWNFRT QDATHGNSLS HRVPGSIGQN QTPGKVFKGK K MAGQMGNE RVTVQSLDVV RVDAERNLLL VKGAVPGATG SDLIVKPAVK A

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Macromolecule #5: 50S ribosomal protein L4

MacromoleculeName: 50S ribosomal protein L4 / type: protein_or_peptide / ID: 5 / Details: Gene Name(s): rplD eryA b3319 JW3281 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (strain K12) (bacteria) / Strain: K12
Molecular weightTheoretical: 22.121566 KDa
SequenceString: MELVLKDAQS ALTVSETTFG RDFNEALVHQ VVVAYAAGAR QGTRAQKTRA EVTGSGKKPW RQKGTGRARS GSIKSPIWRS GGVTFAARP QDHSQKVNKK MYRGALKSIL SELVRQDRLI VVEKFSVEAP KTKLLAQKLK DMALEDVLII TGELDENLFL A ARNLHKVD ...String:
MELVLKDAQS ALTVSETTFG RDFNEALVHQ VVVAYAAGAR QGTRAQKTRA EVTGSGKKPW RQKGTGRARS GSIKSPIWRS GGVTFAARP QDHSQKVNKK MYRGALKSIL SELVRQDRLI VVEKFSVEAP KTKLLAQKLK DMALEDVLII TGELDENLFL A ARNLHKVD VRDATGIDPV SLIAFDKVVM TADAVKQVEE MLA

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Macromolecule #6: 50S ribosomal protein L5

MacromoleculeName: 50S ribosomal protein L5 / type: protein_or_peptide / ID: 6 / Details: Gene Name(s): rplE b3308 JW3270 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (strain K12) (bacteria) / Strain: K12
Molecular weightTheoretical: 20.333611 KDa
SequenceString:
MAKLHDYYKD EVVKKLMTEF NYNSVMQVPR VEKITLNMGV GEAIADKKLL DNAAADLAAI SGQKPLITKA RKSVAGFKIR QGYPIGCKV TLRGERMWEF FERLITIAVP RIRDFRGLSA KSFDGRGNYS MGVREQIIFP EIDYDKVDRV RGLDITITTT A KSDEEGRA LLAAFDFPFR K

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Macromolecule #7: 50S ribosomal protein L6

MacromoleculeName: 50S ribosomal protein L6 / type: protein_or_peptide / ID: 7 / Details: Gene Name(s): rplF b3305 JW3267 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (strain K12) (bacteria) / Strain: K12
Molecular weightTheoretical: 18.932791 KDa
SequenceString:
MSRVAKAPVV VPAGVDVKIN GQVITIKGKN GELTRTLNDA VEVKHADNTL TFGPRDGYAD GWAQAGTARA LLNSMVIGVT EGFTKKLQL VGVGYRAAVK GNVINLSLGF SHPVDHQLPA GITAECPTQT EIVLKGADKQ VIGQVAADLR AYRRPEPYKG K GVRYADEV VRTKEAKKK

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Macromolecule #8: 50S ribosomal protein L9

MacromoleculeName: 50S ribosomal protein L9 / type: protein_or_peptide / ID: 8 / Details: Gene Name(s): rplI b4203 JW4161 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (strain K12) (bacteria) / Strain: K12
Molecular weightTheoretical: 15.78902 KDa
SequenceString:
MQVILLDKVA NLGSLGDQVN VKAGYARNFL VPQGKAVPAT KKNIEFFEAR RAELEAKLAE VLAAANARAE KINALETVTI ASKAGDEGK LFGSIGTRDI ADAVTAAGVE VAKSEVRLPN GVLRTTGEHE VSFQVHSEVF AKVIVNVVAE

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Macromolecule #9: 50S ribosomal protein L11

MacromoleculeName: 50S ribosomal protein L11 / type: protein_or_peptide / ID: 9 / Details: Gene Name(s): rplK relC b3983 JW3946 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (strain K12) (bacteria) / Strain: K12
Molecular weightTheoretical: 14.894362 KDa
SequenceString:
MAKKVQAYVK LQVAAGMANP SPPVGPALGQ QGVNIMEFCK AFNAKTDSIE KGLPIPVVIT VYADRSFTFV TKTPPAAVLL KKAAGIKSG SGKPNKDKVG KISRAQLQEI AQTKAADMTG ADIEAMTRSI EGTARSMGLV VED

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Macromolecule #10: 50S ribosomal protein L13

MacromoleculeName: 50S ribosomal protein L13 / type: protein_or_peptide / ID: 10 / Details: Gene Name(s): rplM b3231 JW3200 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (strain K12) (bacteria) / Strain: K12
Molecular weightTheoretical: 16.050606 KDa
SequenceString:
MKTFTAKPET VKRDWYVVDA TGKTLGRLAT ELARRLRGKH KAEYTPHVDT GDYIIVLNAD KVAVTGNKRT DKVYYHHTGH IGGIKQATF EEMIARRPER VIEIAVKGML PKGPLGRAMF RKLKVYAGNE HNHAAQQPQV LDI

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Macromolecule #11: 50S ribosomal protein L14

MacromoleculeName: 50S ribosomal protein L14 / type: protein_or_peptide / ID: 11 / Details: Gene Name(s): rplN b3310 JW3272 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (strain K12) (bacteria) / Strain: K12
Molecular weightTheoretical: 13.565067 KDa
SequenceString:
MIQEQTMLNV ADNSGARRVM CIKVLGGSHR RYAGVGDIIK ITIKEAIPRG KVKKGDVLKA VVVRTKKGVR RPDGSVIRFD GNACVLLNN NSEQPIGTRI FGPVTRELRS EKFMKIISLA PEVL

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Macromolecule #12: 50S ribosomal protein L15

MacromoleculeName: 50S ribosomal protein L15 / type: protein_or_peptide / ID: 12 / Details: Gene Name(s): rplO b3301 JW3263 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (strain K12) (bacteria) / Strain: K12
Molecular weightTheoretical: 15.008471 KDa
SequenceString:
MRLNTLSPAE GSKKAGKRLG RGIGSGLGKT GGRGHKGQKS RSGGGVRRGF EGGQMPLYRR LPKFGFTSRK AAITAEIRLS DLAKVEGGV VDLNTLKAAN IIGIQIEFAK VILAGEVTTP VTVRGLRVTK GARAAIEAAG GKIEE

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Macromolecule #13: 50S ribosomal protein L16

MacromoleculeName: 50S ribosomal protein L16 / type: protein_or_peptide / ID: 13 / Details: Gene Name(s): rplP b3313 JW3275 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (strain K12) (bacteria) / Strain: K12
Molecular weightTheoretical: 15.312269 KDa
SequenceString:
MLQPKRTKFR KMHKGRNRGL AQGTDVSFGS FGLKAVGRGR LTARQIEAAR RAMTRAVKRQ GKIWIRVFPD KPITEKPLAV RMGKGKGNV EYWVALIQPG KVLYEMDGVP EELAREAFKL AAAKLPIKTT FVTKTVM

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Macromolecule #14: 50S ribosomal protein L17

MacromoleculeName: 50S ribosomal protein L17 / type: protein_or_peptide / ID: 14 / Details: Gene Name(s): rplQ b3294 JW3256 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (strain K12) (bacteria) / Strain: K12
Molecular weightTheoretical: 14.393657 KDa
SequenceString:
MRHRKSGRQL NRNSSHRQAM FRNMAGSLVR HEIIKTTLPK AKELRRVVEP LITLAKTDSV ANRRLAFART RDNEIVAKLF NELGPRFAS RAGGYTRILK CGFRAGDNAP MAYIELVDRS EKAEAAAE

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Macromolecule #15: 50S ribosomal protein L18

MacromoleculeName: 50S ribosomal protein L18 / type: protein_or_peptide / ID: 15 / Details: Gene Name(s): rplR b3304 JW3266 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (strain K12) (bacteria) / Strain: K12
Molecular weightTheoretical: 12.794668 KDa
SequenceString:
MDKKSARIRR ATRARRKLQE LGATRLVVHR TPRHIYAQVI APNGSEVLVA ASTVEKAIAE QLKYTGNKDA AAAVGKAVAE RALEKGIKD VSFDRSGFQY HGRVQALADA AREAGLQF

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Macromolecule #16: 50S ribosomal protein L19

MacromoleculeName: 50S ribosomal protein L19 / type: protein_or_peptide / ID: 16 / Details: Gene Name(s): rplS b2606 JW2587 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (strain K12) (bacteria) / Strain: K12
Molecular weightTheoretical: 13.159278 KDa
SequenceString:
MSNIIKQLEQ EQMKQDVPSF RPGDTVEVKV WVVEGSKKRL QAFEGVVIAI RNRGLHSAFT VRKISNGEGV ERVFQTHSPV VDSISVKRR GAVRKAKLYY LRERTGKAAR IKERLN

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Macromolecule #17: 50S ribosomal protein L20

MacromoleculeName: 50S ribosomal protein L20 / type: protein_or_peptide / ID: 17 / Details: Gene Name(s): rplT pdzA b1716 JW1706 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (strain K12) (bacteria) / Strain: K12
Molecular weightTheoretical: 13.528024 KDa
SequenceString:
MARVKRGVIA RARHKKILKQ AKGYYGARSR VYRVAFQAVI KAGQYAYRDR RQRKRQFRQL WIARINAAAR QNGISYSKFI NGLKKASVE IDRKILADIA VFDKVAFTAL VEKAKAALA

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Macromolecule #18: 50S ribosomal protein L21

MacromoleculeName: 50S ribosomal protein L21 / type: protein_or_peptide / ID: 18 / Details: Gene Name(s): rplU b3186 JW3153 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (strain K12) (bacteria) / Strain: K12
Molecular weightTheoretical: 11.586374 KDa
SequenceString:
MYAVFQSGGK QHRVSEGQTV RLEKLDIATG ETVEFAEVLM IANGEEVKIG VPFVDGGVIK AEVVAHGRGE KVKIVKFRRR KHYRKQQGH RQWFTDVKIT GISA

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Macromolecule #19: 50S ribosomal protein L22

MacromoleculeName: 50S ribosomal protein L22 / type: protein_or_peptide / ID: 19 / Details: Gene Name(s): rplV eryB b3315 JW3277 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (strain K12) (bacteria) / Strain: K12
Molecular weightTheoretical: 12.253359 KDa
SequenceString:
METIAKHRHA RSSAQKVRLV ADLIRGKKVS QALDILTYTN KKAAVLVKKV LESAIANAEH NDGADIDDLK VTKIFVDEGP SMKRIMPRA KGRADRILKR TSHITVVVSD R

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Macromolecule #20: 50S ribosomal protein L23

MacromoleculeName: 50S ribosomal protein L23 / type: protein_or_peptide / ID: 20 / Details: Gene Name(s): rplW b3318 JW3280 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (strain K12) (bacteria) / Strain: K12
Molecular weightTheoretical: 11.22216 KDa
SequenceString:
MIREERLLKV LRAPHVSEKA STAMEKSNTI VLKVAKDATK AEIKAAVQKL FEVEVEVVNT LVVKGKVKRH GQRIGRRSDW KKAYVTLKE GQNLDFVGGA E

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Macromolecule #21: 50S ribosomal protein L24

MacromoleculeName: 50S ribosomal protein L24 / type: protein_or_peptide / ID: 21 / Details: Gene Name(s): rplX b3309 JW3271 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (strain K12) (bacteria) / Strain: K12
Molecular weightTheoretical: 11.33925 KDa
SequenceString:
MAAKIRRDDE VIVLTGKDKG KRGKVKNVLS SGKVIVEGIN LVKKHQKPVP ALNQPGGIVE KEAAIQVSNV AIFNAATGKA DRVGFRFED GKKVRFFKSN SETIK

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Macromolecule #22: 50S ribosomal protein L25

MacromoleculeName: 50S ribosomal protein L25 / type: protein_or_peptide / ID: 22 / Details: Gene Name(s): rplY b2185 JW2173 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (strain K12) (bacteria) / Strain: K12
Molecular weightTheoretical: 10.713465 KDa
SequenceString:
MFTINAEVRK EQGKGASRRL RAANKFPAII YGGKEAPLAI ELDHDKVMNM QAKAEFYSEV LTIVVDGKEI KVKAQDVQRH PYKPKLQHI DFVRA

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Macromolecule #23: 50S ribosomal protein L27

MacromoleculeName: 50S ribosomal protein L27 / type: protein_or_peptide / ID: 23 / Details: Gene Name(s): rpmA b3185 JW3152 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (strain K12) (bacteria) / Strain: K12
Molecular weightTheoretical: 9.14654 KDa
SequenceString:
MAHKKAGGST RNGRDSEAKR LGVKRFGGES VLAGSIIVRQ RGTKFHAGAN VGCGRDHTLF AKADGKVKFE VKGPKNRKFI SIEAE

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Macromolecule #24: 50S ribosomal protein L28

MacromoleculeName: 50S ribosomal protein L28 / type: protein_or_peptide / ID: 24 / Details: Gene Name(s): rpmB b3637 JW3612 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (strain K12) (bacteria) / Strain: K12
Molecular weightTheoretical: 9.027551 KDa
SequenceString:
MSRVCQVTGK RPVTGNNRSH ALNATKRRFL PNLHSHRFWV ESEKRFVTLR VSAKGMRVID KKGIDTVLAE LRARGEKY

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Macromolecule #25: 50S ribosomal protein L29

MacromoleculeName: 50S ribosomal protein L29 / type: protein_or_peptide / ID: 25 / Details: Gene Name(s): rpmC b3312 JW3274 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (strain K12) (bacteria) / Strain: K12
Molecular weightTheoretical: 7.286464 KDa
SequenceString:
MKAKELREKS VEELNTELLN LLREQFNLRM QAASGQLQQS HLLKQVRRDV ARVKTLLNEK AGA

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Macromolecule #26: 50S ribosomal protein L30

MacromoleculeName: 50S ribosomal protein L30 / type: protein_or_peptide / ID: 26 / Details: Gene Name(s): rpmD b3302 JW3264 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli) / Strain: K12
Molecular weightTheoretical: 6.55482 KDa
SequenceString:
MAKTIKITQT RSAIGRLPKH KATLLGLGLR RIGHTVERED TPAIRGMINA VSFMVKVEE

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Macromolecule #27: 50S ribosomal protein L32

MacromoleculeName: 50S ribosomal protein L32 / type: protein_or_peptide / ID: 27 / Details: Gene Name(s): rpmF b1089 JW1075 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli) / Strain: K12
Molecular weightTheoretical: 6.463445 KDa
SequenceString:
MAVQQNKPTR SKRGMRRSHD ALTAVTSLSV DKTSGEKHLR HHITADGYYR GRKVIAK

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Macromolecule #28: 50S ribosomal protein L33

MacromoleculeName: 50S ribosomal protein L33 / type: protein_or_peptide / ID: 28 / Details: Gene Name(s): rpmG b3636 JW3611 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli) / Strain: K12
Molecular weightTheoretical: 6.388631 KDa
SequenceString:
MAKGIREKIK LVSSAGTGHF YTTTKNKRTK PEKLELKKFD PVVRQHVIYK EAKIK

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Macromolecule #29: 50S ribosomal protein L34

MacromoleculeName: 50S ribosomal protein L34 / type: protein_or_peptide / ID: 29 / Details: Gene Name(s): rpmH rimA ssaF b3703 JW3680 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli) / Strain: K12
Molecular weightTheoretical: 5.397463 KDa
SequenceString:
MKRTFQPSVL KRNRSHGFRA RMATKNGRQV LARRRAKGRA RLTVSK

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Macromolecule #30: 50S ribosomal protein L35

MacromoleculeName: 50S ribosomal protein L35 / type: protein_or_peptide / ID: 30 / Details: Gene Name(s): rpmI b1717 JW1707 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli) / Strain: K12
Molecular weightTheoretical: 7.313032 KDa
SequenceString:
MPKIKTVRGA AKRFKKTGKG GFKHKHANLR HILTKKATKR KRHLRPKAMV SKGDLGLVIA CLPYA

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Macromolecule #31: 50S ribosomal protein L36

MacromoleculeName: 50S ribosomal protein L36 / type: protein_or_peptide / ID: 31 / Details: Gene Name(s): rpmJ b3299 JW3261 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli) / Strain: K12
Molecular weightTheoretical: 4.37739 KDa
SequenceString:
MKVRASVKKL CRNCKIVKRD GVIRVICSAE PKHKQRQG

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Macromolecule #33: 30S ribosomal protein S2

MacromoleculeName: 30S ribosomal protein S2 / type: protein_or_peptide / ID: 33 / Details: Gene Name(s): rpsB b0169 JW0164 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli) / Strain: K12
Molecular weightTheoretical: 26.78167 KDa
SequenceString: MATVSMRDML KAGVHFGHQT RYWNPKMKPF IFGARNKVHI INLEKTVPMF NEALAELNKI ASRKGKILFV GTKRAASEAV KDAALSCDQ FFVNHRWLGG MLTNWKTVRQ SIKRLKDLET QSQDGTFDKL TKKEALMRTR ELEKLENSLG GIKDMGGLPD A LFVIDADH ...String:
MATVSMRDML KAGVHFGHQT RYWNPKMKPF IFGARNKVHI INLEKTVPMF NEALAELNKI ASRKGKILFV GTKRAASEAV KDAALSCDQ FFVNHRWLGG MLTNWKTVRQ SIKRLKDLET QSQDGTFDKL TKKEALMRTR ELEKLENSLG GIKDMGGLPD A LFVIDADH EHIAIKEANN LGIPVFAIVD TNSDPDGVDF VIPGNDDAIR AVTLYLGAVA ATVREGRSQD LASQAEESFV EA E

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Macromolecule #34: 30S ribosomal protein S3

MacromoleculeName: 30S ribosomal protein S3 / type: protein_or_peptide / ID: 34 / Details: Gene Name(s): rpsC b3314 JW3276 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli) / Strain: K12
Molecular weightTheoretical: 26.031316 KDa
SequenceString: MGQKVHPNGI RLGIVKPWNS TWFANTKEFA DNLDSDFKVR QYLTKELAKA SVSRIVIERP AKSIRVTIHT ARPGIVIGKK GEDVEKLRK VVADIAGVPA QINIAEVRKP ELDAKLVADS ITSQLERRVM FRRAMKRAVQ NAMRLGAKGI KVEVSGRLGG A EIARTEWY ...String:
MGQKVHPNGI RLGIVKPWNS TWFANTKEFA DNLDSDFKVR QYLTKELAKA SVSRIVIERP AKSIRVTIHT ARPGIVIGKK GEDVEKLRK VVADIAGVPA QINIAEVRKP ELDAKLVADS ITSQLERRVM FRRAMKRAVQ NAMRLGAKGI KVEVSGRLGG A EIARTEWY REGRVPLHTL RADIDYNTSE AHTTYGVIGV KVWIFKGEIL GGMAAVEQPE KPAAQPKKQQ RKGRK

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Macromolecule #35: 30S ribosomal protein S4

MacromoleculeName: 30S ribosomal protein S4 / type: protein_or_peptide / ID: 35 / Details: Gene Name(s): rpsD ramA b3296 JW3258 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli) / Strain: K12
Molecular weightTheoretical: 23.514199 KDa
SequenceString: MARYLGPKLK LSRREGTDLF LKSGVRAIDT KCKIEQAPGQ HGARKPRLSD YGVQLREKQK VRRIYGVLER QFRNYYKEAA RLKGNTGEN LLALLEGRLD NVVYRMGFGA TRAEARQLVS HKAIMVNGRV VNIASYQVSP NDVVSIREKA KKQSRVKAAL E LAEQREKP ...String:
MARYLGPKLK LSRREGTDLF LKSGVRAIDT KCKIEQAPGQ HGARKPRLSD YGVQLREKQK VRRIYGVLER QFRNYYKEAA RLKGNTGEN LLALLEGRLD NVVYRMGFGA TRAEARQLVS HKAIMVNGRV VNIASYQVSP NDVVSIREKA KKQSRVKAAL E LAEQREKP TWLEVDAGKM EGTFKRKPER SDLSADINEH LIVELYSK

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Macromolecule #36: 30S ribosomal protein S5

MacromoleculeName: 30S ribosomal protein S5 / type: protein_or_peptide / ID: 36 / Details: Gene Name(s): rpsE spc b3303 JW3265 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli) / Strain: K12
Molecular weightTheoretical: 17.629398 KDa
SequenceString:
MAHIEKQAGE LQEKLIAVNR VSKTVKGGRI FSFTALTVVG DGNGRVGFGY GKAREVPAAI QKAMEKARRN MINVALNNGT LQHPVKGVH TGSRVFMQPA SEGTGIIAGG AMRAVLEVAG VHNVLAKAYG STNPINVVRA TIDGLENMNS PEMVAAKRGK S VEEILGK

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Macromolecule #37: 30S ribosomal protein S6

MacromoleculeName: 30S ribosomal protein S6 / type: protein_or_peptide / ID: 37 / Details: Gene Name(s): rpsF b4200 JW4158 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (strain K12) (bacteria) / Strain: K12
Molecular weightTheoretical: 15.727512 KDa
SequenceString:
MRHYEIVFMV HPDQSEQVPG MIERYTAAIT GAEGKIHRLE DWGRRQLAYP INKLHKAHYV LMNVEAPQEV IDELETTFRF NDAVIRSMV MRTKHAVTEA SPMVKAKDER RERRDDFANE TADDAEAGDS EEEEEE

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Macromolecule #38: 30S ribosomal protein S7

MacromoleculeName: 30S ribosomal protein S7 / type: protein_or_peptide / ID: 38 / Details: Gene Name(s): rpsG b3341 JW3303 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (strain K12) (bacteria) / Strain: K12
Molecular weightTheoretical: 20.055156 KDa
SequenceString:
MPRRRVIGQR KILPDPKFGS ELLAKFVNIL MVDGKKSTAE SIVYSALETL AQRSGKSELE AFEVALENVR PTVEVKSRRV GGSTYQVPV EVRPVRRNAL AMRWIVEAAR KRGDKSMALR LANELSDAAE NKGTAVKKRE DVHRMAEANK AFAHYRWLSL R SFSHQAGA SSKQPALGYL N

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Macromolecule #39: 30S ribosomal protein S8

MacromoleculeName: 30S ribosomal protein S8 / type: protein_or_peptide / ID: 39 / Details: Gene Name(s): rpsH b3306 JW3268 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli) / Strain: K12
Molecular weightTheoretical: 14.015361 KDa
SequenceString:
SMQDPIADML TRIRNGQAAN KAAVTMPSSK LKVAIANVLK EEGFIEDFKV EGDTKPELEL TLKYFQGKAV VESIQRVSRP GLRIYKRKD ELPKVMAGLG IAVVSTSKGV MTDRAARQAG LGGEIICYVA

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Macromolecule #40: 30S ribosomal protein S9

MacromoleculeName: 30S ribosomal protein S9 / type: protein_or_peptide / ID: 40 / Details: Gene Name(s): rpsI b3230 JW3199 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli) / Strain: K12
Molecular weightTheoretical: 14.88627 KDa
SequenceString:
MAENQYYGTG RRKSSAARVF IKPGNGKIVI NQRSLEQYFG RETARMVVRQ PLELVDMVEK LDLYITVKGG GISGQAGAIR HGITRALME YDESLRSELR KAGFVTRDAR QVERKKVGLR KARRRPQFSK R

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Macromolecule #41: 30S ribosomal protein S10

MacromoleculeName: 30S ribosomal protein S10 / type: protein_or_peptide / ID: 41 / Details: Gene Name(s): rpsJ nusE b3321 JW3283 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli) / Strain: K12
Molecular weightTheoretical: 11.755597 KDa
SequenceString:
MQNQRIRIRL KAFDHRLIDQ ATAEIVETAK RTGAQVRGPI PLPTRKERFT VLISPHVNKD ARDQYEIRTH LRLVDIVEPT EKTVDALMR LDLAAGVDVQ ISLG

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Macromolecule #42: 30S ribosomal protein S11

MacromoleculeName: 30S ribosomal protein S11 / type: protein_or_peptide / ID: 42 / Details: Gene Name(s): rpsK b3297 JW3259 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli) / Strain: K12
Molecular weightTheoretical: 12.4872 KDa
SequenceString:
RKQVSDGVAH IHASFNNTIV TITDRQGNAL GWATAGGSGF RGSRKSTPFA AQVAAERCAD AVKEYGIKNL EVMVKGPGPG RESTIRALN AAGFRITNIT DVTPIPHNGC RPPKKRRV

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Macromolecule #43: 30S ribosomal protein S12

MacromoleculeName: 30S ribosomal protein S12 / type: protein_or_peptide / ID: 43 / Details: Gene Name(s): rpsL strA b3342 JW3304 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli) / Strain: K12
Molecular weightTheoretical: 13.636961 KDa
SequenceString:
ATVNQLVRKP RARKVAKSNV PALEACPQKR GVCTRVYTTT PKKPNSALRK VCRVRLTNGF EVTSYIGGEG HNLQEHSVIL IRGGRVKDL PGVRYHTVRG ALDCSGVKDR KQARSKYGVK RPKA

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Macromolecule #44: 30S ribosomal protein S13

MacromoleculeName: 30S ribosomal protein S13 / type: protein_or_peptide / ID: 44 / Details: Gene Name(s): rpsM b3298 JW3260 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli) / Strain: K12
Molecular weightTheoretical: 13.128467 KDa
SequenceString:
MARIAGINIP DHKHAVIALT SIYGVGKTRS KAILAAAGIA EDVKISELSE GQIDTLRDEV AKFVVEGDLR REISMSIKRL MDLGCYRGL RHRRGLPVRG QRTKTNARTR KGPRKPIKK

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Macromolecule #45: 30S ribosomal protein S14

MacromoleculeName: 30S ribosomal protein S14 / type: protein_or_peptide / ID: 45 / Details: Gene Name(s): rpsN b3307 JW3269 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli) / Strain: K12
Molecular weightTheoretical: 11.475364 KDa
SequenceString:
AKQSMKAREV KRVALADKYF AKRAELKAII SDVNASDEDR WNAVLKLQTL PRDSSPSRQR NRCRQTGRPH GFLRKFGLSR IKVREAAMR GEIPGLKKAS W

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Macromolecule #46: 30S ribosomal protein S15

MacromoleculeName: 30S ribosomal protein S15 / type: protein_or_peptide / ID: 46 / Details: Gene Name(s): rpsO secC b3165 JW3134 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli) / Strain: K12
Molecular weightTheoretical: 10.159621 KDa
SequenceString:
SLSTEATAKI VSEFGRDAND TGSTEVQVAL LTAQINHLQG HFAEHKKDHH SRRGLLRMVS QRRKLLDYLK RKDVARYTQL IERLGLRR

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Macromolecule #47: 30S ribosomal protein S16

MacromoleculeName: 30S ribosomal protein S16 / type: protein_or_peptide / ID: 47 / Details: Gene Name(s): rpsP b2609 JW2590 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli) / Strain: K12
Molecular weightTheoretical: 9.207572 KDa
SequenceString:
MVTIRLARHG AKKRPFYQVV VADSRNARNG RFIERVGFFN PIASEKEEGT RLDLDRIAHW VGQGATISDR VAALIKEVNK AA

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Macromolecule #48: 30S ribosomal protein S17

MacromoleculeName: 30S ribosomal protein S17 / type: protein_or_peptide / ID: 48 / Details: Gene Name(s): rpsQ neaA b3311 JW3273 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli) / Strain: K12
Molecular weightTheoretical: 9.724491 KDa
SequenceString:
MTDKIRTLQG RVVSDKMEKS IVVAIERFVK HPIYGKFIKR TTKLHVHDEN NECGIGDVVE IRECRPLSKT KSWTLVRVVE KAVL

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Macromolecule #49: 30S ribosomal protein S18

MacromoleculeName: 30S ribosomal protein S18 / type: protein_or_peptide / ID: 49 / Details: Gene Name(s): rpsR b4202 JW4160 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli) / Strain: K12
Molecular weightTheoretical: 9.063508 KDa
SequenceString:
MARYFRRRKF CRFTEEGVQE IDYKDIATLK NYITESGKIV PSRITGTRAK YQRQLARAIK RARYLSLLPY TDRHQ

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Macromolecule #50: 30S ribosomal protein S19

MacromoleculeName: 30S ribosomal protein S19 / type: protein_or_peptide / ID: 50 / Details: Gene Name(s): rpsS b3316 JW3278 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli) / Strain: K12
Molecular weightTheoretical: 9.349939 KDa
SequenceString:
PRSLKKGPFI DLHLLKKVEK AVESGDKKPL RTWSRRSTIF PNMIGLTIAV HNGRQHVPVF VTDEMVGHKL GEFAPTRTYR GH

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Macromolecule #51: 30S ribosomal protein S20

MacromoleculeName: 30S ribosomal protein S20 / type: protein_or_peptide / ID: 51 / Details: Gene Name(s): rpsT b0023 JW0022 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli) / Strain: K12
Molecular weightTheoretical: 9.577268 KDa
SequenceString:
ANIKSAKKRA IQSEKARKHN ASRRSMMRTF IKKVYAAIEA GDKAAAQKAF NEMQPIVDRQ AAKGLIHKNK AARHKANLTA QINKLA

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Macromolecule #52: 30S ribosomal protein S21

MacromoleculeName: 30S ribosomal protein S21 / type: protein_or_peptide / ID: 52 / Details: Gene Name(s): rpsU b3065 JW3037 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli) / Strain: K12
Molecular weightTheoretical: 8.524039 KDa
SequenceString:
MPVIKVRENE PFDVALRRFK RSCEKAGVLA EVRRREFYEK PTTERKRAKA SAVKRHAKKL ARENARRTRL Y

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Macromolecule #53: Peptide chain release factor 2

MacromoleculeName: Peptide chain release factor 2 / type: protein_or_peptide / ID: 53 / Details: Gene Name(s): prfB ECK2886 JW5847 supK / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (strain K12) (bacteria) / Strain: K12
Molecular weightTheoretical: 43.313871 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: HHHHHHSAAL EVLFQGPGMF EINPVNNRIQ DLTERSDVLR GYLDYDAKKE RLEEVNAELE QPDVWNEPER AQALGKERSS LEAVVDTLD QMKQGLEDVS GLLELAVEAD DEETFNEAVA ELDALEEKLA QLEFRRMFSG EYDSADCYLD IQAGSGGTEA Q DWASMLER ...String:
HHHHHHSAAL EVLFQGPGMF EINPVNNRIQ DLTERSDVLR GYLDYDAKKE RLEEVNAELE QPDVWNEPER AQALGKERSS LEAVVDTLD QMKQGLEDVS GLLELAVEAD DEETFNEAVA ELDALEEKLA QLEFRRMFSG EYDSADCYLD IQAGSGGTEA Q DWASMLER MYLRWAESRG FKTEIIEESE GEVAGIKSVT IKISGDYAYG WLRTETGVHR LVRKSPFDSG GRRHTSFSSA FV YPEVDDD IDIEINPADL RIDVYRTSGA GG(MEQ)HVNRTES AVRITHIPTG IVTQCQNDRS QHKNKDQAMK QMKAKLYEL EMQKKNAEKQ AMEDNKSDIG WGSQIRSYVL DDSRIKDLRT GVETRNTQAV LDGSLDQFIE ASLKAGL

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Macromolecule #54: Alternative ribosome-rescue factor A

MacromoleculeName: Alternative ribosome-rescue factor A / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (strain K12) (bacteria) / Strain: K12
Molecular weightTheoretical: 6.513396 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString:
GSMSRYQHTK GQIKDNAIEA LLHDPLFRQR VEKNKKGKGS YMRKGKHGNR GNWEASG

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Macromolecule #57: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 57 / Number of copies: 124 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #58: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 58 / Number of copies: 1 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration0.2 mg/mL
BufferpH: 7.5
Component:
ConcentrationFormulaName
20.0 mMC8H18N2O4S4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid
15.0 mMMg(CH3COO)2magnesium acetate
150.0 mMCH3CO2Kpotassium acetate
4.0 mMHOCH2CH2SH2-mercapthoethanol
2.0 mMC7H19N3spermidine
0.05 mMC10H26N4spermin
GridModel: C-flat-2/0.5 4C / Material: COPPER / Mesh: 400 / Pretreatment - Type: GLOW DISCHARGE
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeJEOL 3200FSC
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsCalibrated magnification: 83822 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 3.0 µm / Nominal defocus min: 0.7 µm
Sample stageCooling holder cryogen: NITROGEN
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: SUPER-RESOLUTION / Digitization - Frames/image: 4-25 / Average exposure time: 6.0 sec. / Average electron dose: 20.0 e/Å2

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Image processing

CTF correctionSoftware - Name: CTFFIND (ver. 4)
Startup modelType of model: EMDB MAP
EMDB ID:
Initial angle assignmentType: ANGULAR RECONSTITUTION / Software - Name: RELION (ver. 2.0)
Final angle assignmentType: ANGULAR RECONSTITUTION / Software - Name: RELION (ver. 2.0)
Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.5 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 2.0) / Number images used: 155440
FSC plot (resolution estimation)

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Atomic model buiding 1

RefinementSpace: RECIPROCAL / Protocol: FLEXIBLE FIT / Target criteria: Average FSC
Output model

PDB-5u4i:
Structural Basis of Co-translational Quality Control by ArfA and RF2 Bound to Ribosome

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