5U1C

Structure of tetrameric HIV-1 Strand Transfer Complex Intasome

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, F, G (A, B, C, D)	HIV-1 Integrase, Sso7d chimera	polymer	383	42320.3	4	UniProt (A0A157T5S7) UniProt (F2WR39) Pfam (PF02294) UniProt (by SIFTS) (P39476)	Sulfolobus solfataricus	7 kDa DNA-binding protein d, Sso7d
2	C, H (G, H)	DNA (11-MER)	polymer	11	3349.2	2		Homo sapiens
3	D, I (E, I)	DNA (23-MER)	polymer	23	7015.5	2		Human immunodeficiency virus 1
4	E, J (F, J)	DNA (37-MER)	polymer	37	11414.4	2		Human immunodeficiency virus 1
5	K, M (A, C)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
6	L, N (A, C)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

Sequence modifications

A, B, C, D: -74 - -11 (UniProt: A0A157T5S7)

PDB	External Database	Details
Met -94	-	expression tag
Gly -93	-	expression tag
Ser -92	-	expression tag
Ser -91	-	expression tag
His -90	-	expression tag
His -89	-	expression tag
His -88	-	expression tag
His -87	-	expression tag
His -86	-	expression tag
His -85	-	expression tag
Ser -84	-	expression tag
Ser -83	-	expression tag
Gly -82	-	expression tag
Leu -81	-	expression tag
Val -80	-	expression tag
Pro -79	-	expression tag
Arg -78	-	expression tag
Gly -77	-	expression tag
Ser -76	-	expression tag
His -75	-	expression tag
Gly -10	-	linker
Gly -9	-	linker
Gly -8	-	linker
Gly -7	-	linker
Gly -6	-	linker
Gly -5	-	linker
Gly -4	-	linker
Gly -3	-	linker
Gly -2	-	linker
Gly -1	-	linker
Gly 0	-	linker

A, B, C, D: 1 - 288 (UniProt: F2WR39)

PDB	External Database	Details
Gln 152	Glu 152	engineered mutation

F, J: 1 - 21 (PDB: 5U1C)
F, J: 22 - 37 (PDB: 5U1C)

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	212839.3
Non-Polymers*	Number of molecules	4
	Total formula weight	179.4
All*	Total formula weight	213018.7

*Water molecules are not included.