5TZ2

Crystal structure of human CD47 ECD bound to Fab of C47B222

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (H)	C47B222 Fab Heavy Chain	polymer	229	24643.6	1		Homo sapiens
2	B (L)	C47B222 Fab Light Chain	polymer	214	23406.1	1		Homo sapiens
3	C (C)	Leukocyte surface antigen CD47	polymer	129	14764.5	1	UniProt (Q08722) Pfam (PF08204)	Homo sapiens (Human)	Antigenic surface determinant protein OA3,Integrin-associated protein,IAP,Protein MER6
4	D, E (A, B)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	2	In PDB GlyTouCan (G42666HT)
5	F (L)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
6	G, H, I (C)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	3	Chemie (NAG)
7	J, K, L (H, L, C)	water	water		18.0	105	Chemie (HOH)

Sequence modifications

C: 1 - 123 (UniProt: Q08722)

PDB	External Database	Details
Gly 15	Cys 33	engineered mutation
His 124	-	expression tag
His 125	-	expression tag
His 126	-	expression tag
His 127	-	expression tag
His 128	-	expression tag
His 129	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	62814.2
Branched	Number of molecules	2
	Total formula weight	848.8
Non-Polymers*	Number of molecules	4
	Total formula weight	755.7
All*	Total formula weight	64418.7

*Water molecules are not included.