5TXN
STRUCTURE OF Q151M MUTANT HIV-1 REVERSE TRANSCRIPTASE (RT) TERNARY COMPLEX WITH A DOUBLE STRANDED DNA AND AN INCOMING DATP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, E (A, C) | HIV-1 REVERSE TRANSCRIPTASE P66 SUBUNIT | polymer | 556 | 64025.5 | 2 | UniProt (P03366) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815) Pfam (PF00075) | Human immunodeficiency virus type 1 group M subtype B (isolate BH10) (HIV-1) | Pr160Gag-Pol |
| 2 | B, F (B, D) | HIV-1 REVERSE TRANSCRIPTASE P51 SUBUNIT | polymer | 428 | 50039.5 | 2 | UniProt (P03366) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815) | Human immunodeficiency virus type 1 group M subtype B (isolate BH10) (HIV-1) | Pr160Gag-Pol |
| 3 | C, G (T, E) | DNA (5'-D(*TP*GP*GP*TP*CP*GP*GP*CP*GP*CP*CP*CP*GP*AP*AP*CP*AP*GP*GP*GP*AP*CP*TP*G)-3') | polymer | 27 | 8383.4 | 2 | Human immunodeficiency virus 1 | ||
| 4 | D, H (P, F) | DNA (5'-D(*CP*AP*GP*TP*CP*CP*CP*TP*GP*TP*TP*CP*GP*GP*(MRG)P*CP*GP*CP*CP*G)-3') | polymer | 21 | 6490.3 | 2 | Human immunodeficiency virus 1 | ||
| 5 | I, J (G, H) | beta-D-fructofuranose-(2-1)-alpha-D-glucopyranose | branched | 342.3 | 2 | In PDB BIRD (PRD_900003) | sucrose | ||
| 6 | K, L, P, Q (A, C) | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) | |||
| 7 | M, O (A, C) | 2'-DEOXYADENOSINE 5'-TRIPHOSPHATE | non-polymer | 491.2 | 2 | Chemie (DTP) | |||
| 8 | N, S (B, D) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 2 | Chemie (EDO) | |||
| 9 | R (D) | SULFATE ION | non-polymer | 96.1 | 1 | Chemie (SO4) | |||
| 10 | AA, T, U, V, W... (F, A, B, T, P...) | water | water | 18.0 | 178 | Chemie (HOH) |
Sequence modifications
A, C: 1 - 554 (UniProt: P03366)
B, D: 1 - 428 (UniProt: P03366)
| PDB | External Database | Details |
|---|---|---|
| Met -1 | - | initiating methionine |
| Val 0 | - | expression tag |
| Met 151 | Gln 750 | engineered mutation |
| Cys 258 | Gln 857 | engineered mutation |
| Ser 280 | Cys 879 | engineered mutation |
| Asn 498 | Asp 1097 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ser 280 | Cys 879 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 257877.2 | |
| Branched | Number of molecules | 2 |
| Total formula weight | 684.6 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 1299.8 | |
| All* | Total formula weight | 259861.6 |
