5TXN

STRUCTURE OF Q151M MUTANT HIV-1 REVERSE TRANSCRIPTASE (RT) TERNARY COMPLEX WITH A DOUBLE STRANDED DNA AND AN INCOMING DATP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	104
Detector technology	CCD
Collection date	2013-03-30
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9191
Spacegroup name	P 1 21 1
Unit cell lengths	90.039, 133.634, 139.206
Unit cell angles	90.00, 97.88, 90.00

Refinement procedure

Resolution	33.380 - 2.550
R-factor	0.197
Rwork	0.196
R-free	0.22600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3v4i
RMSD bond length	0.006
RMSD bond angle	0.810
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.550	2.550
Rmerge	0.095
Number of reflections	106245
<I/σ(I)>	9
Completeness [%]	98.1	97
Redundancy	5	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	PEG 8000, AMMONIUM SULFATE, MGCL2, GLYCEROL, SUCROSE