5TWS

Post-catalytic complex of human Polymerase Mu (H329A) with newly incorporated UTP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	human DNA Polymerase Mu	polymer	354	39777.1	1	UniProt (Q9NP87) Pfam (PF14716) Pfam (PF10391) Pfam (PF14792) Pfam (PF14791) In PDB	Homo sapiens	Pol Mu,Terminal transferase
2	T	DNA (5'-D(*CP*GP*GP*CP*AP*TP*AP*CP*G)-3')	polymer	9	2740.8	1		synthetic construct
3	P	DNA/RNA (5'-D(*CP*GP*TP*A)-R(P*U)-3')	polymer	5	1497.0	1		synthetic construct
4	D	DNA (5'-D(P*GP*CP*CP*G)-3')	polymer	4	1191.8	1		synthetic construct
5	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
6	A	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
7	A	GLYCOLIC ACID	non-polymer		76.1	1	Chemie (GOA)
8	A	CHLORIDE ION	non-polymer		35.5	4	Chemie (CL)
9	A	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
10	A	4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID	non-polymer		238.3	1	Chemie (EPE)
11		water	water		18.0	328	Chemie (HOH)

Sequence modifications

A: 134 - 494 (UniProt: Q9NP87)

PDB	External Database	Details
Gly 129	-	expression tag
Ser 130	-	expression tag
Ala 131	-	expression tag
Ala 132	-	expression tag
Ala 133	-	expression tag
Ala 329	His 329	engineered mutation
-	Pro 398	deletion
-	Gly 399	deletion
-	Ala 400	deletion
-	Ala 401	deletion
-	Val 402	deletion
-	Gly 403	deletion
-	Gly 404	deletion
-	Ser 405	deletion
-	Thr 406	deletion
-	Arg 407	deletion
-	Pro 408	deletion
-	Cys 409	deletion
Gly 410	Pro 410	linker

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	45206.7
Non-Polymers*	Number of molecules	10
	Total formula weight	588.5
All*	Total formula weight	45795.2

*Water molecules are not included.