5TWS
Post-catalytic complex of human Polymerase Mu (H329A) with newly incorporated UTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-10-17 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.935, 68.858, 110.319 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.478 - 1.850 |
R-factor | 0.1683 |
Rwork | 0.167 |
R-free | 0.20020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4m04 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.237 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.880 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.331 | |
Number of reflections | 39735 | |
<I/σ(I)> | 45.3 | 2.96 |
Completeness [%] | 99.7 | 96.7 |
Redundancy | 8.4 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 62mM HEPES pH 7.5, 12.4% PEG4K |