5TWQ

Post-catalytic nicked complex of human Polymerase Mu with newly incorporated UTP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	human DNA Polymerase Mu	polymer	354	39844.2	1	UniProt (Q9NP87) Pfam (PF14716) Pfam (PF10391) Pfam (PF14792) Pfam (PF14791) In PDB	Homo sapiens	Pol Mu,Terminal transferase
2	T	DNA (5'-D(*CP*GP*GP*CP*AP*TP*AP*CP*G)-3')	polymer	9	2740.8	1		synthetic construct
3	P	DNA/RNA (5'-D(*CP*GP*TP*A)-R(P*U)-3')	polymer	5	1497.0	1		synthetic construct
4	D	DNA (5'-D(P*GP*CP*CP*G)-3')	polymer	4	1191.8	1		synthetic construct
5	A	MANGANESE (II) ION	non-polymer		54.9	1	Chemie (MN)
6	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
7	A	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
8	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
9	A	PYROPHOSPHATE	non-polymer		178.0	1	Chemie (PPV)
10	A	1,2-ETHANEDIOL	non-polymer		62.1	3	Chemie (EDO)
11		water	water		18.0	365	Chemie (HOH)

Sequence modifications

A: 134 - 494 (UniProt: Q9NP87)

PDB	External Database	Details
Gly 129	-	expression tag
Ser 130	-	expression tag
Ala 131	-	expression tag
Ala 132	-	expression tag
Ala 133	-	expression tag
-	Pro 398	deletion
-	Gly 399	deletion
-	Ala 400	deletion
-	Ala 401	deletion
-	Val 402	deletion
-	Gly 403	deletion
-	Gly 404	deletion
-	Ser 405	deletion
-	Thr 406	deletion
-	Arg 407	deletion
-	Pro 408	deletion
-	Cys 409	deletion
Gly 410	Pro 410	linker

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	45273.8
Non-Polymers*	Number of molecules	8
	Total formula weight	501.9
All*	Total formula weight	45775.7

*Water molecules are not included.