5TR4
Structure of Ubiquitin activating enzyme (Uba1) in complex with ubiquitin and TAK-243
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C (A, C) | Ubiquitin-activating enzyme E1 1 | polymer | 1017 | 113705.2 | 2 | UniProt (P22515) Pfam (PF00899) Pfam (PF16190) Pfam (PF16191) Pfam (PF10585) Pfam (PF09358) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | |
| 2 | B, D (B, D) | Ubiquitin | polymer | 76 | 8568.8 | 2 | UniProt (P0CG53) Pfam (PF00240) | Bos taurus (Bovine) | |
| 3 | E, F (B, D) | [(1~{R},2~{R},3~{S},4~{R})-2,3-bis(oxidanyl)-4-[[2-[3-(trifluoromethylsulfanyl)phenyl]pyrazolo[1,5-a]pyrimidin-7-yl]amino]cyclopentyl]methyl sulfamate | non-polymer | 519.5 | 2 | Chemie (61T) | |||
| 4 | G, H, I, J (A, B, C, D) | water | water | 18.0 | 404 | Chemie (HOH) |
Sequence modifications
A, C: 9 - 1024 (UniProt: P22515)
B, D: 1 - 76 (UniProt: P0CG53)
| PDB | External Database | Details |
|---|---|---|
| Gly 8 | - | expression tag |
| Met 471 | Asn 471 | engineered mutation |
| Arg 519 | Lys 519 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ser 19 | Pro 19 | conflict |
| Asp 24 | Glu 24 | conflict |
| Ser 28 | Ala 28 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 244547.9 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 1039.0 | |
| All* | Total formula weight | 245587.0 |
