5TOO
Crystal structure of alkaline phosphatase PafA T79S, N100A, K162A, R164A mutant
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Alkaline phosphatase PafA | polymer | 549 | 61120.4 | 1 | UniProt (Q9KJX5) Pfam (PF01663) | Elizabethkingia meningoseptica | AP PafA |
| 2 | B, C, D, E (A) | ZINC ION | non-polymer | 65.4 | 4 | Chemie (ZN) | |||
| 3 | F (A) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 4 | G (A) | water | water | 18.0 | 216 | Chemie (HOH) |
Sequence modifications
A: 21 - 546 (UniProt: Q9KJX5)
| PDB | External Database | Details |
|---|---|---|
| Met 12 | - | initiating methionine |
| Asp 13 | - | expression tag |
| Ile 14 | - | expression tag |
| Gly 15 | - | expression tag |
| Ile 16 | - | expression tag |
| Asp 17 | - | expression tag |
| Ser 18 | - | expression tag |
| Asp 19 | - | expression tag |
| Pro 20 | - | expression tag |
| Ser 79 | Thr 79 | engineered mutation |
| Ala 100 | Asn 100 | engineered mutation |
| Ala 162 | Lys 162 | engineered mutation |
| Ala 164 | Arg 164 | engineered mutation |
| Ile 547 | - | expression tag |
| Glu 548 | - | expression tag |
| Gly 549 | - | expression tag |
| Arg 550 | - | expression tag |
| Ser 551 | - | expression tag |
| Ala 552 | - | expression tag |
| Trp 553 | - | expression tag |
| Ser 554 | - | expression tag |
| His 555 | - | expression tag |
| Pro 556 | - | expression tag |
| Gln 557 | - | expression tag |
| Phe 558 | - | expression tag |
| Glu 559 | - | expression tag |
| Lys 560 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 61120.4 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 297.1 | |
| All* | Total formula weight | 61417.5 |
