5THF

Crystal structure of H3 hemagglutinin with insertion of two amino acids in the 150-loop from the A/Hong Kong/1/1968 (H3N2) influenza virus

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E (A, C, E)	Hemagglutinin HA1 chain	polymer	325	35723.2	3	UniProt (Q91MA7) Pfam (PF00509)	Influenza A virus (strain A/Hong Kong/1/1968 H3N2)
2	B, D, F (B, D, F)	Hemagglutinin HA2 chain	polymer	184	20854.9	3	UniProt (Q91MA7) Pfam (PF00509)	Influenza A virus
3	G, I, K (G, I, K)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	3	In PDB GlyTouCan (G15407YE)
4	H, J, L (H, J, L)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	3	In PDB GlyTouCan (G42666HT)
5	M, N, O, P (A, C, E)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	4	Chemie (NAG)
6	Q, R, S, T, U... (A, B, C, D, E...)	water	water		18.0	370	Chemie (HOH)

Sequence modifications

A, C, E: 11 - 329 (UniProt: Q91MA7)

PDB	External Database	Details
Ala 7	-	expression tag
Asp 8	-	expression tag
Pro 9	-	expression tag
Gly 10	-	expression tag
Ser 157	-	insertion
Lys 157	-	insertion

B, D, F: 1 - 176 (UniProt: Q91MA7)

PDB	External Database	Details
Gly 123	Arg 468	engineered mutation
Ser 177	-	expression tag
Gly 178	-	expression tag
Gly 179	-	expression tag
Gly 180	-	expression tag
Gly 181	-	expression tag
Leu 182	-	expression tag
Asn 183	-	expression tag
Asp 184	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	169734.4
Branched	Number of molecules	6
	Total formula weight	3032.8
Non-Polymers*	Number of molecules	4
	Total formula weight	884.8
All*	Total formula weight	173652.1

*Water molecules are not included.