5THF
Crystal structure of H3 hemagglutinin with insertion of two amino acids in the 150-loop from the A/Hong Kong/1/1968 (H3N2) influenza virus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-27 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.03320 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 68.981, 236.435, 72.645 |
Unit cell angles | 90.00, 116.49, 90.00 |
Refinement procedure
Resolution | 48.601 - 2.590 |
R-factor | 0.1895 |
Rwork | 0.187 |
R-free | 0.23900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4fnk |
RMSD bond length | 0.015 |
RMSD bond angle | 1.993 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.640 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 64033 | |
<I/σ(I)> | 12.7 | 2.2 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 3.7 | 3.6 |
CC(1/2) | 0.670 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293.15 | 20% (w/v) PEG 3500, 0.2M Na2HPO4 pH 9.1 |