5TGS

Crystal Structure of QueE from Bacillus subtilis with methionine bound

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	7-carboxy-7-deazaguanine synthase	polymer	263	29323.3	2	UniProt (O31677) Pfam (PF13353) Pfam (PF04055)	Bacillus subtilis	CDG synthase,Queuosine biosynthesis protein QueE
2	C, F (A, B)	IRON/SULFUR CLUSTER	non-polymer		351.6	2	Chemie (SF4)
3	D (A)	METHIONINE	non-polymer		149.2	1	Chemie (MET)
4	E (A)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
5	G, H (A, B)	water	water		18.0	85	Chemie (HOH)

Sequence modifications

A, B: 1 - 243 (UniProt: O31677)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	58646.6
Non-Polymers*	Number of molecules	4
	Total formula weight	958.6
All*	Total formula weight	59605.2

*Water molecules are not included.