5TGS
Crystal Structure of QueE from Bacillus subtilis with methionine bound
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9999 , 1.7389 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.182, 79.297, 122.224 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.011 - 2.548 |
| R-factor | 0.2173 |
| Rwork | 0.216 |
| R-free | 0.24820 |
| Structure solution method | MAD |
| RMSD bond length | 0.076 |
| RMSD bond angle | 2.128 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.548 | 2.548 |
| Number of reflections | 17745 | |
| <I/σ(I)> | 17.7 | 2.3 |
| Completeness [%] | 99.3 | 98 |
| Redundancy | 3.9 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.1 MTris HCl (pH 8.5), 22% PEG 8000 and 0.1 M Magnesium chloride |
