5TGO

Crystal structure of H10 hemagglutinin mutant (K158aA-D193T-Q226L-G228S) from Jiangxi-Donghu (2013) H10N8 influenza virus

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E	Hemagglutinin HA1 chain	polymer	323	35226.8	3	UniProt (A0A0J9X252) Pfam (PF00509) UniProt (by SIFTS) (A0A059T4A1) In PDB	Influenza A virus
2	B, D, F	Hemagglutinin HA2 chain	polymer	180	20698.8	3	UniProt (A0A0J9X253) Pfam (PF00509) UniProt (by SIFTS) (A0A059T4A1) In PDB	Influenza A virus
3	G	alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		545.5	1	In PDB GlyTouCan (G41734DZ)
4	H	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
5	A, C	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	4	Chemie (NAG)
6		water	water		18.0	589	Chemie (HOH)

Sequence modifications

A, C, E: 7 - 326 (UniProt: A0A0J9X252)

PDB	External Database	Details
Ala 158	Lys 154	engineered mutation
Thr 193	Asp 190	engineered mutation
Leu 226	Gln 223	engineered mutation
Ser 228	Gly 225	engineered mutation

B, D, F: 2 - 174 (UniProt: A0A0J9X253)

PDB	External Database	Details
Ser 175	-	expression tag
Gly 176	-	expression tag
Arg 177	-	expression tag
Leu 178	-	expression tag
Val 179	-	expression tag
Pro 180	-	expression tag
Arg 181	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	167776.5
Branched	Number of molecules	2
	Total formula weight	969.9
Non-Polymers*	Number of molecules	4
	Total formula weight	884.8
All*	Total formula weight	169631.3

*Water molecules are not included.