5TGO

Crystal structure of H10 hemagglutinin mutant (K158aA-D193T-Q226L-G228S) from Jiangxi-Donghu (2013) H10N8 influenza virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.03320
Spacegroup name	P 1 21 1
Unit cell lengths	63.957, 253.931, 69.932
Unit cell angles	90.00, 111.73, 90.00

Refinement procedure

Resolution	37.369 - 2.350
R-factor	0.2021
Rwork	0.200
R-free	0.24190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xq5
RMSD bond length	0.011
RMSD bond angle	2.181
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.390
High resolution limit [Å]	2.350	2.350
Number of reflections	82400
<I/σ(I)>	9.8	1.3
Completeness [%]	95.3
Redundancy	2.3	2.3
CC(1/2)		0.672

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293.15	0.2 M NaCl, 10% (w/v) PEG 8000, 0.1M CHES pH 9.5