5TF6

Structure and conformational plasticity of the U6 small nuclear ribonucleoprotein core

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	U4/U6 snRNA-associated-splicing factor PRP24	polymer	374	43246.8	2	UniProt (P49960) Pfam (PF00076) Pfam (PF16842)	Saccharomyces cerevisiae (Baker's yeast)	U4/U6 snRNP protein
2	B, D (B, D)	U6 snRNA	polymer	72	23168.9	2	GenBank (1039022925)	Saccharomyces cerevisiae
3	E, J, L, M, R (A, B, C, D)	POTASSIUM ION	non-polymer		39.1	5	Chemie (K)
4	F, G, N, O (A, C)	CHLORIDE ION	non-polymer		35.5	4	Chemie (CL)
5	H, I, K, P, Q (A, B, C)	GLYCEROL	non-polymer		92.1	5	Chemie (GOL)
6	S, T, U, V (A, B, C, D)	water	water		18.0	749	Chemie (HOH)

Sequence modifications

A, C: 34 - 400 (UniProt: P49960)

PDB	External Database	Details
Met 33	-	initiating methionine
His 401	-	expression tag
His 402	-	expression tag
His 403	-	expression tag
His 404	-	expression tag
His 405	-	expression tag
His 406	-	expression tag

B, D: 30 - 101 (GenBank: 1039022925)

PDB	External Database	Details
C 100	U 365726	engineered mutation
C 101	U 365727	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	132831.2
Non-Polymers*	Number of molecules	14
	Total formula weight	797.8
All*	Total formula weight	133629.0

*Water molecules are not included.