5TF6

Structure and conformational plasticity of the U6 small nuclear ribonucleoprotein core

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-10-23
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	76.921, 84.446, 255.142
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.100 - 2.300
R-factor	0.1863
Rwork	0.185
R-free	0.23160
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.771
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	49.100
High resolution limit [Å]	2.300
Rmerge	0.090
Number of reflections	141843
<I/σ(I)>	17.67
Completeness [%]	99.7
Redundancy	12.1
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	1 M lithium chloride, 100 mM sodium MES, 64 mM hydrochloric acid, 25% PEG 8,000, 20% glycerol, 1 mM MnCl2, 400 mM potassium chloride, 10 mM HEPES acid, 10 mM Tris base, 2 mM MgCl2, 1 mM TCEP-HCl