5TCH

Crystal structure of tryptophan synthase from M. tuberculosis - ligand-free form, TrpA-G66V mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, G, E, C	Tryptophan synthase alpha chain	polymer	276	28621.5	4	UniProt (P9WFY1) Pfam (PF00290) In PDB	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
2	B, H, F, D	Tryptophan synthase beta chain	polymer	410	43562.7	4	UniProt (P9WFX9) Pfam (PF00291) In PDB	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
3	C, D, A, B, G...	MALONATE ION	non-polymer		102.0	8	Chemie (MLI)
4	F, C, D, A, B...	FORMIC ACID	non-polymer		46.0	20	Chemie (FMT)
5		water	water		18.0	795	Chemie (HOH)

Sequence modifications

A, G, E, C: 1 - 270 (UniProt: P9WFY1)

PDB	External Database	Details
Val 66	Gly 66	engineered mutation
His 271	-	expression tag
His 272	-	expression tag
His 273	-	expression tag
His 274	-	expression tag
His 275	-	expression tag
His 276	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	288737.0
Non-Polymers*	Number of molecules	28
	Total formula weight	1736.9
All*	Total formula weight	290473.8

*Water molecules are not included.