5TCH
Crystal structure of tryptophan synthase from M. tuberculosis - ligand-free form, TrpA-G66V mutant
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000162 | biological_process | L-tryptophan biosynthetic process |
| A | 0004834 | molecular_function | tryptophan synthase activity |
| A | 0005829 | cellular_component | cytosol |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0006568 | biological_process | L-tryptophan metabolic process |
| A | 0008652 | biological_process | amino acid biosynthetic process |
| A | 0009073 | biological_process | aromatic amino acid family biosynthetic process |
| A | 0016829 | molecular_function | lyase activity |
| B | 0000162 | biological_process | L-tryptophan biosynthetic process |
| B | 0004834 | molecular_function | tryptophan synthase activity |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0006568 | biological_process | L-tryptophan metabolic process |
| B | 0008652 | biological_process | amino acid biosynthetic process |
| B | 0009073 | biological_process | aromatic amino acid family biosynthetic process |
| B | 0016829 | molecular_function | lyase activity |
| B | 1901605 | biological_process | alpha-amino acid metabolic process |
| C | 0000162 | biological_process | L-tryptophan biosynthetic process |
| C | 0004834 | molecular_function | tryptophan synthase activity |
| C | 0005829 | cellular_component | cytosol |
| C | 0005886 | cellular_component | plasma membrane |
| C | 0006568 | biological_process | L-tryptophan metabolic process |
| C | 0008652 | biological_process | amino acid biosynthetic process |
| C | 0009073 | biological_process | aromatic amino acid family biosynthetic process |
| C | 0016829 | molecular_function | lyase activity |
| D | 0000162 | biological_process | L-tryptophan biosynthetic process |
| D | 0004834 | molecular_function | tryptophan synthase activity |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0006568 | biological_process | L-tryptophan metabolic process |
| D | 0008652 | biological_process | amino acid biosynthetic process |
| D | 0009073 | biological_process | aromatic amino acid family biosynthetic process |
| D | 0016829 | molecular_function | lyase activity |
| D | 1901605 | biological_process | alpha-amino acid metabolic process |
| E | 0000162 | biological_process | L-tryptophan biosynthetic process |
| E | 0004834 | molecular_function | tryptophan synthase activity |
| E | 0005829 | cellular_component | cytosol |
| E | 0005886 | cellular_component | plasma membrane |
| E | 0006568 | biological_process | L-tryptophan metabolic process |
| E | 0008652 | biological_process | amino acid biosynthetic process |
| E | 0009073 | biological_process | aromatic amino acid family biosynthetic process |
| E | 0016829 | molecular_function | lyase activity |
| F | 0000162 | biological_process | L-tryptophan biosynthetic process |
| F | 0004834 | molecular_function | tryptophan synthase activity |
| F | 0005737 | cellular_component | cytoplasm |
| F | 0006568 | biological_process | L-tryptophan metabolic process |
| F | 0008652 | biological_process | amino acid biosynthetic process |
| F | 0009073 | biological_process | aromatic amino acid family biosynthetic process |
| F | 0016829 | molecular_function | lyase activity |
| F | 1901605 | biological_process | alpha-amino acid metabolic process |
| G | 0000162 | biological_process | L-tryptophan biosynthetic process |
| G | 0004834 | molecular_function | tryptophan synthase activity |
| G | 0005829 | cellular_component | cytosol |
| G | 0005886 | cellular_component | plasma membrane |
| G | 0006568 | biological_process | L-tryptophan metabolic process |
| G | 0008652 | biological_process | amino acid biosynthetic process |
| G | 0009073 | biological_process | aromatic amino acid family biosynthetic process |
| G | 0016829 | molecular_function | lyase activity |
| H | 0000162 | biological_process | L-tryptophan biosynthetic process |
| H | 0004834 | molecular_function | tryptophan synthase activity |
| H | 0005737 | cellular_component | cytoplasm |
| H | 0006568 | biological_process | L-tryptophan metabolic process |
| H | 0008652 | biological_process | amino acid biosynthetic process |
| H | 0009073 | biological_process | aromatic amino acid family biosynthetic process |
| H | 0016829 | molecular_function | lyase activity |
| H | 1901605 | biological_process | alpha-amino acid metabolic process |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 9 |
| Details | binding site for residue MLI A 301 |
| Chain | Residue |
| A | TYR181 |
| A | GLY217 |
| A | LEU218 |
| A | GLY219 |
| A | GLY239 |
| A | SER240 |
| A | FMT302 |
| A | HOH405 |
| A | HOH416 |
| site_id | AC2 |
| Number of Residues | 6 |
| Details | binding site for residue FMT A 302 |
| Chain | Residue |
| A | TYR30 |
| A | GLU57 |
| A | MET106 |
| A | TYR181 |
| A | ILE237 |
| A | MLI301 |
| site_id | AC3 |
| Number of Residues | 7 |
| Details | binding site for residue MLI B 501 |
| Chain | Residue |
| B | THR124 |
| B | GLY125 |
| B | ALA126 |
| B | GLN128 |
| B | HIS129 |
| B | LEU180 |
| B | ALA316 |
| site_id | AC4 |
| Number of Residues | 2 |
| Details | binding site for residue FMT B 502 |
| Chain | Residue |
| B | LYS47 |
| B | GLU48 |
| site_id | AC5 |
| Number of Residues | 3 |
| Details | binding site for residue FMT B 503 |
| Chain | Residue |
| B | ARG265 |
| B | ASP337 |
| B | GLU378 |
| site_id | AC6 |
| Number of Residues | 3 |
| Details | binding site for residue FMT B 504 |
| Chain | Residue |
| A | GLN144 |
| B | SER23 |
| B | TYR29 |
| site_id | AC7 |
| Number of Residues | 7 |
| Details | binding site for residue MLI G 301 |
| Chain | Residue |
| G | TYR181 |
| G | GLY217 |
| G | GLY219 |
| G | GLY239 |
| G | SER240 |
| G | FMT302 |
| G | HOH406 |
| site_id | AC8 |
| Number of Residues | 5 |
| Details | binding site for residue FMT G 302 |
| Chain | Residue |
| G | TYR30 |
| G | GLU57 |
| G | MET106 |
| G | TYR181 |
| G | MLI301 |
| site_id | AC9 |
| Number of Residues | 3 |
| Details | binding site for residue FMT G 303 |
| Chain | Residue |
| G | GLN144 |
| H | SER23 |
| H | TYR29 |
| site_id | AD1 |
| Number of Residues | 6 |
| Details | binding site for residue MLI H 501 |
| Chain | Residue |
| H | THR124 |
| H | GLY125 |
| H | ALA126 |
| H | GLN128 |
| H | HIS129 |
| H | LEU180 |
| site_id | AD2 |
| Number of Residues | 4 |
| Details | binding site for residue FMT H 502 |
| Chain | Residue |
| H | LYS47 |
| H | GLU48 |
| H | ARG114 |
| H | HOH703 |
| site_id | AD3 |
| Number of Residues | 4 |
| Details | binding site for residue FMT H 503 |
| Chain | Residue |
| B | LYS402 |
| B | GLY405 |
| B | GLY408 |
| H | TRP403 |
| site_id | AD4 |
| Number of Residues | 2 |
| Details | binding site for residue FMT H 504 |
| Chain | Residue |
| H | ASP337 |
| H | GLU378 |
| site_id | AD5 |
| Number of Residues | 6 |
| Details | binding site for residue FMT H 505 |
| Chain | Residue |
| B | HOH628 |
| D | ARG164 |
| D | HOH605 |
| H | ARG355 |
| H | PHE404 |
| H | HOH630 |
| site_id | AD6 |
| Number of Residues | 6 |
| Details | binding site for residue MLI E 301 |
| Chain | Residue |
| E | TYR181 |
| E | GLY217 |
| E | GLY219 |
| E | GLY239 |
| E | SER240 |
| E | FMT303 |
| site_id | AD7 |
| Number of Residues | 1 |
| Details | binding site for residue FMT E 302 |
| Chain | Residue |
| E | GLN144 |
| site_id | AD8 |
| Number of Residues | 6 |
| Details | binding site for residue FMT E 303 |
| Chain | Residue |
| E | TYR30 |
| E | GLU57 |
| E | ILE72 |
| E | MET106 |
| E | TYR181 |
| E | MLI301 |
| site_id | AD9 |
| Number of Residues | 8 |
| Details | binding site for residue MLI F 501 |
| Chain | Residue |
| F | LLP101 |
| F | THR124 |
| F | GLY125 |
| F | ALA126 |
| F | GLN128 |
| F | HIS129 |
| F | LEU180 |
| F | ALA316 |
| site_id | AE1 |
| Number of Residues | 3 |
| Details | binding site for residue FMT F 502 |
| Chain | Residue |
| F | LYS47 |
| F | GLU48 |
| F | ARG114 |
| site_id | AE2 |
| Number of Residues | 2 |
| Details | binding site for residue FMT F 503 |
| Chain | Residue |
| D | SER84 |
| F | ARG236 |
| site_id | AE3 |
| Number of Residues | 5 |
| Details | binding site for residue MLI C 301 |
| Chain | Residue |
| C | TYR181 |
| C | GLY217 |
| C | GLY219 |
| C | GLY239 |
| C | SER240 |
| site_id | AE4 |
| Number of Residues | 3 |
| Details | binding site for residue FMT C 302 |
| Chain | Residue |
| C | TYR181 |
| C | ILE237 |
| C | GLU57 |
| site_id | AE5 |
| Number of Residues | 9 |
| Details | binding site for residue MLI D 501 |
| Chain | Residue |
| D | THR124 |
| D | GLY125 |
| D | ALA126 |
| D | GLN128 |
| D | HIS129 |
| D | LEU180 |
| D | ALA316 |
| D | HOH653 |
| D | HOH689 |
| site_id | AE6 |
| Number of Residues | 4 |
| Details | binding site for residue FMT D 502 |
| Chain | Residue |
| D | ALA36 |
| D | GLU40 |
| D | ALA195 |
| D | ASP196 |
| site_id | AE7 |
| Number of Residues | 2 |
| Details | binding site for residue FMT D 503 |
| Chain | Residue |
| D | GLU48 |
| D | ARG114 |
| site_id | AE8 |
| Number of Residues | 4 |
| Details | binding site for residue FMT D 504 |
| Chain | Residue |
| D | HIS11 |
| D | LEU259 |
| D | ASP260 |
| D | ARG335 |
| site_id | AE9 |
| Number of Residues | 2 |
| Details | binding site for residue FMT D 505 |
| Chain | Residue |
| C | GLN144 |
| D | SER23 |
| site_id | AF1 |
| Number of Residues | 3 |
| Details | binding site for residue FMT D 506 |
| Chain | Residue |
| D | ARG119 |
| D | HOH667 |
| H | GLN80 |
Functional Information from PROSITE/UniProt
| site_id | PS00167 |
| Number of Residues | 14 |
| Details | TRP_SYNTHASE_ALPHA Tryptophan synthase alpha chain signature. IEVGvPYSDPVMDG |
| Chain | Residue | Details |
| A | ILE56-GLY69 |
| site_id | PS00168 |
| Number of Residues | 15 |
| Details | TRP_SYNTHASE_BETA Tryptophan synthase beta chain pyridoxal-phosphate attachment site. LnHtGSHKiNnvLgQ |
| Chain | Residue | Details |
| B | LEU94-GLN108 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 8 |
| Details | Active site: {"description":"Proton acceptor","evidences":[{"source":"HAMAP-Rule","id":"MF_00131","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | Modified residue: {"description":"N6-(pyridoxal phosphate)lysine","evidences":[{"source":"HAMAP-Rule","id":"MF_00133","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
