5T8K

1.95 Angstrom Crystal Structure of S-adenosylhomocysteinase from Cryptosporidium parvum in Complex with Adenine and NAD

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, D (A, B, D)	Adenosylhomocysteinase	polymer	498	56200.7	3	UniProt (Q5CPH1) Pfam (PF05221) Pfam (PF00670)	Cryptosporidium parvum (strain Iowa II)
2	C (C)	Adenosylhomocysteinase	polymer	498	56276.8	1	UniProt (Q5CPH1) Pfam (PF05221) Pfam (PF00670)	Cryptosporidium parvum (strain Iowa II)
3	E, K, Q, X (A, B, C, D)	SODIUM ION	non-polymer		23.0	4	Chemie (NA)
4	F, L, R, Y (A, B, C, D)	NICOTINAMIDE-ADENINE-DINUCLEOTIDE	non-polymer		663.4	4	Chemie (NAD)
5	G, M, S, Z (A, B, C, D)	ADENINE	non-polymer		135.1	4	Chemie (ADE)
6	H, N, T, U (A, B, C)	HEXAETHYLENE GLYCOL	non-polymer		282.3	4	Chemie (P6G)
7	I (A)	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	1	Chemie (TRS)
8	J, P (A, B)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
9	CA, O, V, W (D, B, C)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	4	Chemie (PEG)
10	AA, BA (D)	TRIETHYLENE GLYCOL	non-polymer		150.2	2	Chemie (PGE)
11	DA, EA, FA, GA (A, B, C, D)	water	water		18.0	1925	Chemie (HOH)

Sequence modifications

A, B, D: 1 - 495 (UniProt: Q5CPH1)

PDB	External Database	Details
Ser -2	-	expression tag
Asn -1	-	expression tag
Ala 0	-	expression tag

C: 1 - 495 (UniProt: Q5CPH1)

PDB	External Database	Details
Ser -2	-	expression tag
Asn -1	-	expression tag
Ala 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	224878.8
Non-Polymers*	Number of molecules	25
	Total formula weight	5446.6
All*	Total formula weight	230325.5

*Water molecules are not included.