5T8K
1.95 Angstrom Crystal Structure of S-adenosylhomocysteinase from Cryptosporidium parvum in Complex with Adenine and NAD
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0004013 | molecular_function | adenosylhomocysteinase activity |
| A | 0005829 | cellular_component | cytosol |
| A | 0006730 | biological_process | one-carbon metabolic process |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0033353 | biological_process | S-adenosylmethionine cycle |
| B | 0000166 | molecular_function | nucleotide binding |
| B | 0004013 | molecular_function | adenosylhomocysteinase activity |
| B | 0005829 | cellular_component | cytosol |
| B | 0006730 | biological_process | one-carbon metabolic process |
| B | 0016787 | molecular_function | hydrolase activity |
| B | 0033353 | biological_process | S-adenosylmethionine cycle |
| C | 0000166 | molecular_function | nucleotide binding |
| C | 0004013 | molecular_function | adenosylhomocysteinase activity |
| C | 0005829 | cellular_component | cytosol |
| C | 0006730 | biological_process | one-carbon metabolic process |
| C | 0016787 | molecular_function | hydrolase activity |
| C | 0033353 | biological_process | S-adenosylmethionine cycle |
| D | 0000166 | molecular_function | nucleotide binding |
| D | 0004013 | molecular_function | adenosylhomocysteinase activity |
| D | 0005829 | cellular_component | cytosol |
| D | 0006730 | biological_process | one-carbon metabolic process |
| D | 0016787 | molecular_function | hydrolase activity |
| D | 0033353 | biological_process | S-adenosylmethionine cycle |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 6 |
| Details | binding site for residue NA A 501 |
| Chain | Residue |
| A | GLU57 |
| A | THR408 |
| A | GLY409 |
| A | HIS410 |
| A | ADE503 |
| A | HOH717 |
| site_id | AC2 |
| Number of Residues | 32 |
| Details | binding site for residue NAD A 502 |
| Chain | Residue |
| A | ASN246 |
| A | GLY275 |
| A | GLY277 |
| A | GLU278 |
| A | VAL279 |
| A | THR297 |
| A | GLU298 |
| A | ILE299 |
| A | ASP300 |
| A | CYS303 |
| A | THR331 |
| A | GLY332 |
| A | ASN333 |
| A | VAL336 |
| A | ILE354 |
| A | GLY355 |
| A | HIS356 |
| A | ASN403 |
| A | HIS410 |
| A | ADE503 |
| A | HOH625 |
| A | HOH731 |
| A | HOH744 |
| A | HOH824 |
| A | HOH827 |
| A | HOH872 |
| A | HOH928 |
| C | LYS489 |
| C | TYR493 |
| A | THR212 |
| A | THR213 |
| A | THR214 |
| site_id | AC3 |
| Number of Residues | 12 |
| Details | binding site for residue ADE A 503 |
| Chain | Residue |
| A | LEU52 |
| A | HIS53 |
| A | THR55 |
| A | GLU57 |
| A | THR58 |
| A | THR408 |
| A | GLY409 |
| A | HIS410 |
| A | MET415 |
| A | NA501 |
| A | NAD502 |
| A | HOH622 |
| site_id | AC4 |
| Number of Residues | 3 |
| Details | binding site for residue P6G A 504 |
| Chain | Residue |
| A | LYS36 |
| A | GLU452 |
| A | TYR456 |
| site_id | AC5 |
| Number of Residues | 3 |
| Details | binding site for residue TRS A 505 |
| Chain | Residue |
| A | ILE7 |
| A | LYS8 |
| A | TRP101 |
| site_id | AC6 |
| Number of Residues | 4 |
| Details | binding site for residue GOL A 506 |
| Chain | Residue |
| A | TYR206 |
| A | ARG433 |
| A | GLU434 |
| A | VAL436 |
| site_id | AC7 |
| Number of Residues | 6 |
| Details | binding site for residue NA B 501 |
| Chain | Residue |
| B | GLU57 |
| B | THR408 |
| B | GLY409 |
| B | HIS410 |
| B | ADE503 |
| B | HOH880 |
| site_id | AC8 |
| Number of Residues | 32 |
| Details | binding site for residue NAD B 502 |
| Chain | Residue |
| B | THR212 |
| B | THR213 |
| B | THR214 |
| B | ASN246 |
| B | GLY275 |
| B | GLY277 |
| B | GLU278 |
| B | VAL279 |
| B | THR297 |
| B | GLU298 |
| B | ILE299 |
| B | ASP300 |
| B | CYS303 |
| B | THR331 |
| B | GLY332 |
| B | ASN333 |
| B | VAL336 |
| B | ILE354 |
| B | GLY355 |
| B | HIS356 |
| B | ASN403 |
| B | HIS410 |
| B | ADE503 |
| B | HOH624 |
| B | HOH776 |
| B | HOH785 |
| B | HOH792 |
| B | HOH793 |
| B | HOH863 |
| B | HOH925 |
| D | LYS489 |
| D | TYR493 |
| site_id | AC9 |
| Number of Residues | 12 |
| Details | binding site for residue ADE B 503 |
| Chain | Residue |
| B | HIS53 |
| B | THR55 |
| B | GLU57 |
| B | THR58 |
| B | THR408 |
| B | GLY409 |
| B | HIS410 |
| B | MET415 |
| B | NA501 |
| B | NAD502 |
| B | HOH620 |
| B | LEU52 |
| site_id | AD1 |
| Number of Residues | 6 |
| Details | binding site for residue P6G B 504 |
| Chain | Residue |
| B | ASN481 |
| B | PRO487 |
| B | LYS489 |
| B | SER490 |
| B | HOH806 |
| D | SER224 |
| site_id | AD2 |
| Number of Residues | 3 |
| Details | binding site for residue PEG B 505 |
| Chain | Residue |
| B | LYS36 |
| B | TYR37 |
| B | HOH889 |
| site_id | AD3 |
| Number of Residues | 9 |
| Details | binding site for residue GOL B 506 |
| Chain | Residue |
| B | ARG218 |
| B | PHE357 |
| B | ASP359 |
| B | HOH643 |
| B | HOH718 |
| B | HOH736 |
| B | HOH740 |
| B | HOH854 |
| D | TYR479 |
| site_id | AD4 |
| Number of Residues | 6 |
| Details | binding site for residue NA C 501 |
| Chain | Residue |
| C | GLU57 |
| C | THR408 |
| C | GLY409 |
| C | HIS410 |
| C | ADE503 |
| C | HOH859 |
| site_id | AD5 |
| Number of Residues | 33 |
| Details | binding site for residue NAD C 502 |
| Chain | Residue |
| A | LYS489 |
| A | TYR493 |
| C | THR212 |
| C | THR213 |
| C | THR214 |
| C | ASN246 |
| C | GLY275 |
| C | GLY277 |
| C | GLU278 |
| C | VAL279 |
| C | THR297 |
| C | GLU298 |
| C | ILE299 |
| C | ASP300 |
| C | CYS303 |
| C | THR331 |
| C | GLY332 |
| C | ASN333 |
| C | VAL336 |
| C | ILE354 |
| C | GLY355 |
| C | HIS356 |
| C | LEU401 |
| C | ASN403 |
| C | HIS410 |
| C | ADE503 |
| C | HOH712 |
| C | HOH734 |
| C | HOH812 |
| C | HOH831 |
| C | HOH861 |
| C | HOH877 |
| C | HOH954 |
| site_id | AD6 |
| Number of Residues | 12 |
| Details | binding site for residue ADE C 503 |
| Chain | Residue |
| C | LEU52 |
| C | HIS53 |
| C | THR55 |
| C | GLU57 |
| C | THR58 |
| C | THR408 |
| C | GLY409 |
| C | HIS410 |
| C | MET415 |
| C | NA501 |
| C | NAD502 |
| C | HOH670 |
| site_id | AD7 |
| Number of Residues | 7 |
| Details | binding site for residue P6G C 504 |
| Chain | Residue |
| A | SER224 |
| C | ASN481 |
| C | VAL482 |
| C | SER483 |
| C | PRO487 |
| C | LYS489 |
| C | HOH878 |
| site_id | AD8 |
| Number of Residues | 4 |
| Details | binding site for residue P6G C 505 |
| Chain | Residue |
| C | LYS36 |
| C | TYR37 |
| C | LEU453 |
| C | HOH880 |
| site_id | AD9 |
| Number of Residues | 1 |
| Details | binding site for residue PEG C 506 |
| Chain | Residue |
| C | ILE7 |
| site_id | AE1 |
| Number of Residues | 4 |
| Details | binding site for residue PEG C 507 |
| Chain | Residue |
| C | TYR206 |
| C | ARG433 |
| C | GLU434 |
| C | VAL436 |
| site_id | AE2 |
| Number of Residues | 6 |
| Details | binding site for residue NA D 501 |
| Chain | Residue |
| D | GLU57 |
| D | THR408 |
| D | GLY409 |
| D | HIS410 |
| D | ADE503 |
| D | HOH950 |
| site_id | AE3 |
| Number of Residues | 33 |
| Details | binding site for residue NAD D 502 |
| Chain | Residue |
| B | LYS489 |
| B | TYR493 |
| D | THR212 |
| D | THR213 |
| D | THR214 |
| D | ASN246 |
| D | GLY275 |
| D | GLY277 |
| D | GLU278 |
| D | VAL279 |
| D | THR297 |
| D | GLU298 |
| D | ILE299 |
| D | ASP300 |
| D | CYS303 |
| D | THR331 |
| D | GLY332 |
| D | ASN333 |
| D | VAL336 |
| D | ILE354 |
| D | GLY355 |
| D | HIS356 |
| D | ASN403 |
| D | HIS410 |
| D | ADE503 |
| D | HOH629 |
| D | HOH771 |
| D | HOH792 |
| D | HOH797 |
| D | HOH802 |
| D | HOH862 |
| D | HOH872 |
| D | HOH929 |
| site_id | AE4 |
| Number of Residues | 12 |
| Details | binding site for residue ADE D 503 |
| Chain | Residue |
| D | LEU52 |
| D | HIS53 |
| D | THR55 |
| D | GLU57 |
| D | THR58 |
| D | THR408 |
| D | GLY409 |
| D | HIS410 |
| D | MET415 |
| D | NA501 |
| D | NAD502 |
| D | HOH624 |
| site_id | AE5 |
| Number of Residues | 3 |
| Details | binding site for residue PGE D 504 |
| Chain | Residue |
| D | LYS36 |
| D | TYR37 |
| D | TYR456 |
| site_id | AE6 |
| Number of Residues | 2 |
| Details | binding site for residue PGE D 505 |
| Chain | Residue |
| D | ASN481 |
| D | SER490 |
| site_id | AE7 |
| Number of Residues | 3 |
| Details | binding site for residue PEG D 506 |
| Chain | Residue |
| D | ILE7 |
| D | TRP101 |
| D | HOH736 |
Functional Information from PROSITE/UniProt
| site_id | PS00738 |
| Number of Residues | 15 |
| Details | ADOHCYASE_1 S-adenosyl-L-homocysteine hydrolase signature 1. SCNiYSTQDhAAAAL |
| Chain | Residue | Details |
| A | SER76-LEU90 | |
| C | SER76-LEU90 |
| site_id | PS00739 |
| Number of Residues | 17 |
| Details | ADOHCYASE_2 S-adenosyl-L-homocysteine hydrolase signature 2. GKkivVlGYGeVGKGc.A |
| Chain | Residue | Details |
| A | GLY268-ALA284 | |
| C | GLY268-ALA284 |
