5OYJ

Crystal structure of VEGFR-2 domains 4-5 in complex with DARPin D4b

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	DARPin D4b	polymer	169	18339.4	2		synthetic construct
2	C, D (C, D)	Vascular endothelial growth factor receptor 2	polymer	234	26253.7	2	UniProt (P35968) Pfam (PF07679) Pfam (PF22971)	Homo sapiens (Human)	VEGFR-2,Fetal liver kinase 1,FLK-1,Kinase insert domain receptor,KDR,Protein-tyrosine kinase receptor flk-1
3	E, F, G, H, I... (E, F, G, H, I...)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	7	In PDB GlyTouCan (G42666HT)
4	L, M, N (L, M, N)	alpha-D-glucopyranose-(1-1)-alpha-D-glucopyranose	branched		342.3	3	In PDB BIRD (PRD_900006) GlyTouCan (G92130SN)		trehalose
5	O, P, Q, S (A, C)	CALCIUM ION	non-polymer		40.1	4	Chemie (CA)
6	R (A)	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
7	T (C)	CACODYLATE ION	non-polymer		137.0	1	Chemie (CAC)
8	U (C)	ACETATE ION	non-polymer		59.0	1	Chemie (ACT)
9	V, W, X, Y (A, B, C, D)	water	water		18.0	270	Chemie (HOH)

Sequence modifications

C, D: 326 - 549 (UniProt: P35968)

PDB	External Database	Details
Ala 322	-	expression tag
Pro 323	-	expression tag
Leu 324	-	expression tag
Ala 325	-	expression tag
His 550	-	expression tag
His 551	-	expression tag
His 552	-	expression tag
His 553	-	expression tag
His 554	-	expression tag
His 555	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	89186.3
Branched	Number of molecules	10
	Total formula weight	3997.7
Non-Polymers*	Number of molecules	7
	Total formula weight	379.3
All*	Total formula weight	93563.3

*Water molecules are not included.