5OYJ

Crystal structure of VEGFR-2 domains 4-5 in complex with DARPin D4b

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-05-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	109.489, 164.559, 70.759
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.749 - 2.380
R-factor	0.1804
Rwork	0.178
R-free	0.22880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4bsj 2qyj
RMSD bond length	0.009
RMSD bond angle	1.060
Data reduction software	XDS
Data scaling software	Aimless (0.5.26)
Phasing software	PHASER
Refinement software	PHENIX ((dev_2722: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.900	2.440
High resolution limit [Å]	2.380	2.380
Rmerge	0.136	2.370
Rpim	0.038	0.680
Number of reflections	52035	3808
<I/σ(I)>	10.7	1.3
Completeness [%]	99.9	100
Redundancy	13.2	13
CC(1/2)	0.998	0.675

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	293.15	0.2 M calcium acetate monohydrate, 15% PEG 4000, 0.1 M sodium cacodylate pH 6.4, cryoprotected with 15% trehalose