5OX9

Structure of the Cyan Fluorescent Protein SCFP3A at pH 4.5

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Green fluorescent protein	polymer	245	27682.2	1	UniProt (P42212) Pfam (PF01353) In PDB	Aequorea victoria (Jellyfish)
2	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
3		water	water		18.0	156	Chemie (HOH)

Sequence modifications

A: 2 - 238 (UniProt: P42212)

PDB	External Database	Details
Met 0	-	initiating methionine
Val 1	-	expression tag
Leu 64	Phe 64	engineered mutation
B2h 66	Ser 65	chromophore
B2h 66	Tyr 66	chromophore
B2h 66	Gly 67	chromophore
Ala 72	Ser 72	engineered mutation
Ile 146	Asn 146	engineered mutation
Asp 148	His 148	engineered mutation
Thr 153	Met 153	engineered mutation
Ala 163	Val 163	engineered mutation
Gly 175	Ser 175	engineered mutation
Leu 231	His 231	conflict
His 239	-	expression tag
His 240	-	expression tag
His 241	-	expression tag
His 242	-	expression tag
His 243	-	expression tag
His 244	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	27682.2
Non-Polymers*	Number of molecules	1
	Total formula weight	35.5
All*	Total formula weight	27717.7

*Water molecules are not included.