5OX9
Structure of the Cyan Fluorescent Protein SCFP3A at pH 4.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-09 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.87260 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.875, 60.396, 69.295 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.530 - 1.560 |
| R-factor | 0.14462 |
| Rwork | 0.143 |
| R-free | 0.17233 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wso |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.349 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.530 | 1.600 |
| High resolution limit [Å] | 1.560 | 1.560 |
| Rmeas | 0.067 | 0.773 |
| Number of reflections | 31581 | 3106 |
| <I/σ(I)> | 12.2 | 2 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 100mM citric acid pH4.5, 16% PEG8000, 100mM MgCl2 |
