5OT8
Structure of the periplasmic binding protein (PBP) NocT-G97S mutant from A. tumefaciens C58 in complex with octopine.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Nopaline-binding periplasmic protein | polymer | 265 | 29289.7 | 2 | UniProt (P35120) Pfam (PF00497) | Agrobacterium fabrum str. C58 | |
| 2 | C, R (A, B) | octopine | non-polymer | 246.3 | 2 | Chemie (6DB) | |||
| 3 | D, E, F (A) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 3 | Chemie (EDO) | |||
| 4 | G (A) | SULFATE ION | non-polymer | 96.1 | 1 | Chemie (SO4) | |||
| 5 | H, I, J, K, L... (A, B) | CHLORIDE ION | non-polymer | 35.5 | 9 | Chemie (CL) | |||
| 6 | O (A) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 1 | Chemie (PEG) | |||
| 7 | S, T (A, B) | water | water | 18.0 | 51 | Chemie (HOH) |
Sequence modifications
A, B: 26 - 283 (UniProt: P35120)
| PDB | External Database | Details |
|---|---|---|
| Met 25 | - | initiating methionine |
| Ser 97 | Gly 97 | engineered mutation |
| His 284 | - | expression tag |
| His 285 | - | expression tag |
| His 286 | - | expression tag |
| His 287 | - | expression tag |
| His 288 | - | expression tag |
| His 289 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 58579.3 | |
| Non-Polymers* | Number of molecules | 16 |
| Total formula weight | 1200.0 | |
| All* | Total formula weight | 59779.3 |
