5OQ9
XFEL structure of the off state of a reversibly photoswitching fluorescent protein determined using the droplet on demand injection method
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Green to red photoconvertible GFP-like protein EosFP | polymer | 226 | 25724.3 | 1 | UniProt (Q5S6Z9) Pfam (PF01353) | Lobophyllia hemprichii (Lobed brain coral) | |
| 2 | B (A) | water | water | 18.0 | 207 | Chemie (HOH) |
Sequence modifications
A: 1 - 226 (UniProt: Q5S6Z9)
| PDB | External Database | Details |
|---|---|---|
| Lys 11 | Asn 11 | engineered mutation |
| Vya 62 | His 62 | chromophore |
| Vya 62 | Gly 64 | chromophore |
| Lys 70 | Glu 70 | engineered mutation |
| Asn 74 | His 74 | engineered mutation |
| Tyr 121 | His 121 | engineered mutation |
| Thr 123 | Val 123 | engineered mutation |
| Val 157 | Ile 157 | engineered mutation |
| Glu 158 | Thr 158 | engineered mutation |
| Ala 189 | Tyr 189 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 25724.3 | |
| All* | Total formula weight | 25724.3 |
