5OQ9
XFEL structure of the off state of a reversibly photoswitching fluorescent protein determined using the droplet on demand injection method
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SACLA BEAMLINE BL3 |
| Synchrotron site | SACLA |
| Beamline | BL3 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2016-07-16 |
| Detector | MPCCD |
| Wavelength(s) | 1.18 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.800, 74.600, 79.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.300 - 1.510 |
| R-factor | 0.178 |
| Rwork | 0.175 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.180 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 54.300 |
| High resolution limit [Å] | 1.450 |
| Number of reflections | 37792 |
| <I/σ(I)> | 6.84 |
| Completeness [%] | 90.4 |
| Redundancy | 11.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 293 | 0.2 M lithium sulfate, 0.1 M Tris-Cl pH 8.5, 23-30% PEG 3350, |
