5OAJ

Crystal structure of mutant AChBP in complex with tropisetron (T53F, Q74R, Y110A, I135S, G162E)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	Soluble acetylcholine receptor	polymer	249	28355.8	10	UniProt (Q8WSF8) Pfam (PF02931) In PDB	Aplysia californica (California sea hare)
2	E, A, H, I	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	4	Chemie (NAG)
3	D, I, E, J, F...	1,2-ETHANEDIOL	non-polymer		62.1	42	Chemie (EDO)
4	G, C, I	CITRATE ANION	non-polymer		189.1	3	Chemie (FLC)
5	E, J	(3-ENDO)-8-METHYL-8-AZABICYCLO[3.2.1]OCT-3-YL 1H-INDOLE-3-CARBOXYLATE	non-polymer		284.4	2	Chemie (TKT)
6		water	water		18.0	541	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F, G, H, I, J: 1 - 236 (UniProt: Q8WSF8)

PDB	External Database	Details
Phe 53	Thr 53	engineered mutation
Val 60	Ala 60	conflict
Arg 74	Gln 74	engineered mutation
Ala 110	Tyr 110	engineered mutation
Ser 135	Ile 135	engineered mutation
Val 155	Ala 155	conflict
Glu 162	Gly 162	engineered mutation
Glu 237	-	expression tag
Asn 238	-	expression tag
Leu 239	-	expression tag
Tyr 240	-	expression tag
Phe 241	-	expression tag
Gln 242	-	expression tag
Gly 243	-	expression tag
His 244	-	expression tag
His 245	-	expression tag
His 246	-	expression tag
His 247	-	expression tag
His 248	-	expression tag
His 249	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	283557.7
Non-Polymers*	Number of molecules	51
	Total formula weight	4627.7
All*	Total formula weight	288185.4

*Water molecules are not included.