5O9J

Crystal structure of transcription factor IIB Mja mini-intein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Transcription initiation factor IIB,Transcription initiation factor IIB	polymer	184	20872.1	2	UniProt (Q58192) Pfam (PF14890)	Methanocaldococcus jannaschii	TFIIB,TFIIB
2	C, D, E, F, P... (A, B)	1,4-DIETHYLENE DIOXIDE	non-polymer		88.1	6	Chemie (DIO)
3	AA, BA, CA, G, H... (B, A)	GLYCEROL	non-polymer		92.1	14	Chemie (GOL)
4	DA, EA, FA, GA, J... (B, A)	AMMONIUM ION	non-polymer		18.0	10	Chemie (NH4)
5	R (B)	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	1	Chemie (MES)
6	HA, IA (A, B)	water	water		18.0	194	Chemie (HOH)

Sequence modifications

A, B: 2 - 126 (UniProt: Q58192)

PDB	External Database	Details
Gly -2	-	expression tag
Gly -1	-	expression tag
Tyr 0	-	expression tag
Ala 1	-	expression tag
Arg 122	Glu 221	conflict
Ile 123	Leu 222	conflict
Thr 124	Pro 223	conflict

A, B: 134 - 181 (UniProt: Q58192)

PDB	External Database	Details
Tyr 127	-	linker
Thr 128	-	linker
Asn 129	-	linker
Arg 130	-	linker
Lys 131	-	linker
Leu 132	-	linker
Glu 133	-	linker
Ala 181	Thr 435	conflict

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	41744.2
Non-Polymers*	Number of molecules	31
	Total formula weight	2193.6
All*	Total formula weight	43937.8

*Water molecules are not included.