5O9J
Crystal structure of transcription factor IIB Mja mini-intein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-07-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97950 |
Spacegroup name | P 1 |
Unit cell lengths | 42.360, 50.798, 60.118 |
Unit cell angles | 77.42, 75.83, 76.93 |
Refinement procedure
Resolution | 28.700 - 2.000 |
R-factor | 0.2153 |
Rwork | 0.214 |
R-free | 0.26120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2cw8 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.013 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MR-Rosetta |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.700 | |
High resolution limit [Å] | 2.000 | 2.070 |
Rmerge | 0.085 | 0.724 |
Number of reflections | 30100 | |
<I/σ(I)> | 11.6 | 1.6 |
Completeness [%] | 95.9 | 92.5 |
Redundancy | 2.4 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294 | MES, dioxane, ammonium sulfate |