5O9A

Crystal structure of the GluA2 ligand-binding domain (S1S2J-L504Y-N775S) in complex with glutamate and BPAM121 at 1.78 A resolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Glutamate receptor 2,Glutamate receptor 2	polymer	264	29301.7	4	UniProt (P19491) Pfam (PF10613) Pfam (PF00060)	Rattus norvegicus (Norway Rat)	GluR-2,AMPA-selective glutamate receptor 2,GluR-B,GluR-K2,Glutamate receptor ionotropic,AMPA 2,GluA2
2	AA, BA, E, F, G... (C, A, D, B)	SULFATE ION	non-polymer		96.1	16	Chemie (SO4)
3	CA, I, MA, S (C, A, D, B)	7-chloro-4-(2-fluoroethyl)-2,3-dihydro-1,2,4-benzothiadiazine 1,1-dioxide	non-polymer		264.7	4	Chemie (7M6)
4	DA, J, K, L, NA... (C, A, D, B)	CHLORIDE ION	non-polymer		35.5	7	Chemie (CL)
5	FA, M, O, U (C, A, B)	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
6	HA, N, PA, V (C, A, D, B)	GLUTAMIC ACID	non-polymer		147.1	4	Chemie (GLU)
7	P (B)	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
8	EA (C)	CITRIC ACID	non-polymer		192.1	1	Chemie (CIT)
9	GA (C)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
10	QA, RA, SA, TA (A, B, C, D)	water	water		18.0	1160	Chemie (HOH)

Sequence modifications

A, B, C, D: 3 - 117 (UniProt: P19491)

PDB	External Database	Details
Gly 1	-	cloning artifact
Ala 2	-	cloning artifact
Tyr 94	Leu 504	engineered mutation

A, B, C, D: 120 - 264 (UniProt: P19491)

PDB	External Database	Details
Gly 118	-	linker
Thr 119	-	linker
Ser 242	Asn 775	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	117206.9
Non-Polymers*	Number of molecules	38
	Total formula weight	4161.2
All*	Total formula weight	121368.1

*Water molecules are not included.