5O8Q

Crystal structure of R. ruber ADH-A, mutant Y294F, W295A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	Alcohol dehydrogenase	polymer	352	35968.9	8	UniProt (A0A1Q8I6M1) In PDB	Rhodococcus sp. M8
2	G, H, A, B, C...	ZINC ION	non-polymer		65.4	16	Chemie (ZN)
3	G, H, A, B, C...	NICOTINAMIDE-ADENINE-DINUCLEOTIDE	non-polymer		663.4	8	Chemie (NAD)
4		water	water		18.0	752	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F, G, H: 1 - 345 (UniProt: A0A1Q8I6M1)

PDB	External Database	Details
Phe 294	Tyr 294	engineered mutation
Ala 295	Trp 295	engineered mutation
Thr 346	-	expression tag
Ser 347	-	expression tag
His 348	-	expression tag
His 349	-	expression tag
His 350	-	expression tag
His 351	-	expression tag
His 352	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	287751.1
Non-Polymers*	Number of molecules	24
	Total formula weight	6353.9
All*	Total formula weight	294105.0

*Water molecules are not included.