5O8F
Structure of a chimaeric beta3-alpha5 GABAA receptor in complex with nanobody Nb25 and pregnanolone
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D, E (A, B, C, D, E) | Gamma-aminobutyric acid receptor subunit beta-3,Gamma-aminobutyric acid receptor subunit alpha-5,Gamma-aminobutyric acid receptor subunit alpha-5 | polymer | 367 | 41667.9 | 5 | UniProt (P28472) UniProt (P31644) Pfam (PF02931) Pfam (PF02932) | Homo sapiens (Human) | GABA(A) receptor subunit beta-3,GABA(A) receptor subunit alpha-5,GABA(A) receptor subunit alpha-5 |
| 2 | F, J (K, O) | Nanobody Nb25 | polymer | 125 | 13673.0 | 2 | Lama glama (Llama) | ||
| 3 | G, H, I (L, M, N) | Nanobody Nb25 | polymer | 124 | 13585.9 | 3 | Lama glama (llama) | ||
| 4 | K, L, M, N, O (F, G, H, I, J) | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1235.1 | 5 | In PDB GlyTouCan (G16404YH) | |||
| 5 | P, Q, S, U, Y (A, B, C, D, E) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 5 | Chemie (NAG) | |||
| 6 | R, T, V, W, X (B, C, D, E) | Pregnanolone | non-polymer | 318.5 | 5 | Chemie (P9N) |
Sequence modifications
A, B, C, D, E: 1 - 229 (UniProt: P28472)
A, B, C, D, E: 230 - 315 (UniProt: P31644)
A, B, C, D, E: 393 - 431 (UniProt: P31644)
| PDB | External Database | Details |
|---|---|---|
| Glu -2 | - | expression tag |
| Thr -1 | - | expression tag |
| Gly 0 | - | expression tag |
A, B, C, D, E: 393 - 431 (UniProt: P31644)
| PDB | External Database | Details |
|---|---|---|
| Ser 316 | - | linker |
| Gln 317 | - | linker |
| Pro 318 | - | linker |
| Ala 319 | - | linker |
| Arg 320 | - | linker |
| Ala 321 | - | linker |
| Ala 322 | - | linker |
| Ile 404 | Val 435 | conflict |
| Gly 432 | - | expression tag |
| Thr 433 | - | expression tag |
| Thr 434 | - | expression tag |
| Glu 435 | - | expression tag |
| Thr 436 | - | expression tag |
| Ser 437 | - | expression tag |
| Gln 438 | - | expression tag |
| Val 439 | - | expression tag |
| Ala 440 | - | expression tag |
| Pro 441 | - | expression tag |
| Ala 442 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 10 |
| Total formula weight | 276443.3 | |
| Branched | Number of molecules | 5 |
| Total formula weight | 6175.5 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 2698.5 | |
| All* | Total formula weight | 285317.4 |
