5O8F
Structure of a chimaeric beta3-alpha5 GABAA receptor in complex with nanobody Nb25 and pregnanolone
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C, D, E | Gamma-aminobutyric acid receptor subunit beta-3,Gamma-aminobutyric acid receptor subunit alpha-5,Gamma-aminobutyric acid receptor subunit alpha-5 | polymer | 367 | 41667.9 | 5 | UniProt (P28472) UniProt (P31644) Pfam (PF02931) Pfam (PF02932) In PDB | Homo sapiens (Human) | GABA(A) receptor subunit beta-3,GABA(A) receptor subunit alpha-5,GABA(A) receptor subunit alpha-5 |
2 | K, O | Nanobody Nb25 | polymer | 125 | 13673.0 | 2 | Lama glama (Llama) | ||
3 | L, M, N | Nanobody Nb25 | polymer | 124 | 13585.9 | 3 | Lama glama (llama) | ||
4 | F, G, H, I, J | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1235.1 | 5 | In PDB GlyTouCan (G16404YH) | |||
5 | A, B, C, D, E | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 5 | Chemie (NAG) | |||
6 | B, C, D, E | Pregnanolone | non-polymer | 318.5 | 5 | Chemie (P9N) |
Sequence modifications
A, B, C, D, E: 1 - 229 (UniProt: P28472)
A, B, C, D, E: 230 - 315 (UniProt: P31644)
A, B, C, D, E: 393 - 431 (UniProt: P31644)
PDB | External Database | Details |
---|---|---|
Glu -2 | - | expression tag |
Thr -1 | - | expression tag |
Gly 0 | - | expression tag |
A, B, C, D, E: 393 - 431 (UniProt: P31644)
PDB | External Database | Details |
---|---|---|
Ser 316 | - | linker |
Gln 317 | - | linker |
Pro 318 | - | linker |
Ala 319 | - | linker |
Arg 320 | - | linker |
Ala 321 | - | linker |
Ala 322 | - | linker |
Ile 404 | Val 435 | conflict |
Gly 432 | - | expression tag |
Thr 433 | - | expression tag |
Thr 434 | - | expression tag |
Glu 435 | - | expression tag |
Thr 436 | - | expression tag |
Ser 437 | - | expression tag |
Gln 438 | - | expression tag |
Val 439 | - | expression tag |
Ala 440 | - | expression tag |
Pro 441 | - | expression tag |
Ala 442 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 10 |
Total formula weight | 276443.3 | |
Branched | Number of molecules | 5 |
Total formula weight | 6175.5 | |
Non-Polymers* | Number of molecules | 10 |
Total formula weight | 2698.5 | |
All* | Total formula weight | 285317.4 |