5O8E

Mutant of class II CPD photolyase from Methanosarcina mazei

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Deoxyribodipyrimidine photolyase	polymer	484	55328.8	1	UniProt (Q8PYK9) Pfam (PF00875) In PDB	Methanosarcina mazei Go1
2	A	FLAVIN-ADENINE DINUCLEOTIDE	non-polymer		785.5	1	Chemie (FAD)
3	A	SULFATE ION	non-polymer		96.1	8	Chemie (SO4)
4	A	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
5		water	water		18.0	523	Chemie (HOH)

Sequence modifications

A: 1 - 464 (UniProt: Q8PYK9)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Phe 360	Trp 360	engineered mutation
Thr 377	Met 377	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	55328.8
Non-Polymers*	Number of molecules	11
	Total formula weight	1738.2
All*	Total formula weight	57067.0

*Water molecules are not included.