5O8E

Mutant of class II CPD photolyase from Methanosarcina mazei

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-08-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.918409
Spacegroup name	P 43 21 2
Unit cell lengths	69.720, 69.720, 244.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.178 - 1.700
R-factor	0.1567
Rwork	0.156
R-free	0.19190
RMSD bond length	0.008
RMSD bond angle	1.001
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	45.950
High resolution limit [Å]	1.700
Rmerge	0.093
Number of reflections	67494
<I/σ(I)>	21.9
Completeness [%]	100.0
Redundancy	12.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	0.5 M LITHIUM SULFATE, 7.5% (W/V) PEG 6000