5O74

Crystal structure of human Rab1b covalently bound to the GEF domain of DrrA/SidM from Legionella pneumophila in the presence of GDP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G, I... (A, C, E, G, I...)	Multifunctional virulence effector protein DrrA	polymer	197	22001.2	6	UniProt (Q29ST3)	Legionella pneumophila	Defects in Rab1 recruitment protein A
2	B, D, F, H, J... (B, D, F, H, J...)	Ras-related protein Rab-1B	polymer	180	20605.1	6	UniProt (Q9H0U4) Pfam (PF00071)	Homo sapiens (Human)
3	M, N, O, P, Q... (B, D, F, H, J...)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	6	Chemie (GDP) PubChem (136073927) PubChem (135398619) PubChem (135402030) PubChem (135431654) PubChem (135566337) PubChem (135760556) PubChem (136026707) PubChem (136053418) PubChem (136663964) PubChem (136664748) PubChem (136759848) PubChem (136759849) PubChem (136759850) PubChem (136836636) PubChem (136847074) PubChem (172898537)	Homo sapiens (Human)

Sequence modifications

A, C, E, G, I, K: 340 - 533 (UniProt: Q29ST3)

PDB	External Database	Details
Gly 337	-	expression tag
His 338	-	expression tag
Met 339	-	expression tag
Cys 512	Asp 512	engineered mutation

B, D, F, H, J, L: 3 - 174 (UniProt: Q9H0U4)

PDB	External Database	Details
Met 1	-	initiating methionine
Ala 2	-	expression tag
9mn 79	Arg 79	engineered mutation
His 175	-	expression tag
His 176	-	expression tag
His 177	-	expression tag
His 178	-	expression tag
His 179	-	expression tag
His 180	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	255638.1
Non-Polymers*	Number of molecules	6
	Total formula weight	2659.2
All*	Total formula weight	258297.3

*Water molecules are not included.