5O74

Crystal structure of human Rab1b covalently bound to the GEF domain of DrrA/SidM from Legionella pneumophila in the presence of GDP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G, I... (A, C, E, G, I...)	Multifunctional virulence effector protein DrrA	polymer	197	22001.2	6	UniProt (Q29ST3) Pfam (PF20851)	Legionella pneumophila	Defects in Rab1 recruitment protein A
2	B, D, F, H, J... (B, D, F, H, J...)	Ras-related protein Rab-1B	polymer	180	20605.1	6	UniProt (Q9H0U4) Pfam (PF00071)	Homo sapiens (Human)
3	M, N, O, P, Q... (B, D, F, H, J...)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	6	Chemie (GDP)	Homo sapiens (Human)

Sequence modifications

A, C, E, G, I, K: 340 - 533 (UniProt: Q29ST3)

PDB	External Database	Details
Gly 337	-	expression tag
His 338	-	expression tag
Met 339	-	expression tag
Cys 512	Asp 512	engineered mutation

B, D, F, H, J, L: 3 - 174 (UniProt: Q9H0U4)

PDB	External Database	Details
Met 1	-	initiating methionine
Ala 2	-	expression tag
9mn 79	Arg 79	engineered mutation
His 175	-	expression tag
His 176	-	expression tag
His 177	-	expression tag
His 178	-	expression tag
His 179	-	expression tag
His 180	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	255638.1
Non-Polymers*	Number of molecules	6
	Total formula weight	2659.2
All*	Total formula weight	258297.3

*Water molecules are not included.