5NV4

UDP-Glucose Glycoprotein Glucosyltransferase from Chaetomium thermophilum double mutant D611C:G1050C

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	UDP-glucose-glycoprotein glucosyltransferase-like protein	polymer	1494	169672.5	1	UniProt (G0SB58) Pfam (PF18400) Pfam (PF18401) Pfam (PF18402) Pfam (PF18403) Pfam (PF06427) Pfam (PF18404) In PDB	Chaetomium thermophilum var. thermophilum DSM 1495
2	B	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	1	In PDB GlyTouCan (G15407YE)
3	A	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	4	Chemie (NAG)
4	A	FORMIC ACID	non-polymer		46.0	1	Chemie (FMT)
5		water	water		18.0	34	Chemie (HOH)

Sequence modifications

A: 24 - 1505 (UniProt: G0SB58)

PDB	External Database	Details
Glu 21	-	expression tag
Thr 22	-	expression tag
Gly 23	-	expression tag
Cys 611	Asp 611	engineered mutation
Cys 1050	Gly 1050	engineered mutation
Gly 1506	-	expression tag
Thr 1507	-	expression tag
Lys 1508	-	expression tag
His 1509	-	expression tag
His 1510	-	expression tag
His 1511	-	expression tag
His 1512	-	expression tag
His 1513	-	expression tag
His 1514	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	169672.5
Branched	Number of molecules	1
	Total formula weight	586.5
Non-Polymers*	Number of molecules	5
	Total formula weight	930.9
All*	Total formula weight	171189.9

*Water molecules are not included.