5NS9

Crystal structure of the GluA2 LBD (L483Y-N754S-L758V) in complex with glutamate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Glutamate receptor 2,Glutamate receptor 2	polymer	292	32344.0	2	UniProt (P19491) Pfam (PF10613) Pfam (PF00060) In PDB	Rattus norvegicus (Norway rat)	GluR-2,AMPA-selective glutamate receptor 2,GluR-B,GluR-K2,Glutamate receptor ionotropic,AMPA 2,GluA2,GluR-2,AMPA-selective glutamate receptor 2,GluR-B,GluR-K2,Glutamate receptor ionotropic,AMPA 2,GluA2
2	A	PENTAETHYLENE GLYCOL	non-polymer		238.3	2	Chemie (1PE)
3	A	2-(2-METHOXYETHOXY)ETHANOL	non-polymer		120.1	1	Chemie (PG0)
4	A, B	SULFATE ION	non-polymer		96.1	6	Chemie (SO4)
5	A, B	SODIUM ION	non-polymer		23.0	5	Chemie (NA)
6	A, B	GLUTAMIC ACID	non-polymer		147.1	2	Chemie (GLU)
7		water	water		18.0	905	Chemie (HOH)

Sequence modifications

A, B: 3 - 117 (UniProt: P19491)

PDB	External Database	Details
Met -27	-	initiating methionine
His -26	-	expression tag
His -25	-	expression tag
His -24	-	expression tag
His -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
Gly -16	-	expression tag
Leu -15	-	expression tag
Val -14	-	expression tag
Pro -13	-	expression tag
Arg -12	-	expression tag
Gly -11	-	expression tag
Ser -10	-	expression tag
Ala -9	-	expression tag
Met -8	-	expression tag
Gly -7	-	expression tag
Ser -6	-	expression tag
Gly -5	-	expression tag
Asn -4	-	expression tag
Asp -3	-	expression tag
Thr -2	-	expression tag
Ser -1	-	expression tag
Arg 0	-	expression tag
Gly 1	-	expression tag
Ala 2	-	expression tag
Tyr 94	Leu 504	engineered mutation

A, B: 120 - 264 (UniProt: P19491)

PDB	External Database	Details
Gly 118	-	linker
Thr 119	-	linker
Ser 242	Asn 775	engineered mutation
Val 246	Leu 779	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	64688.1
Non-Polymers*	Number of molecules	16
	Total formula weight	1582.3
All*	Total formula weight	66270.3

*Water molecules are not included.